ArticleMethods in molecular biology (Clifton, N.J.)2011
The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Bridging technical innovation and computational advances in studies of RNA-protein assemblies.Nature reviews. Genetics · 2026Review
- Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation.Communications biology · 2023Article
- Ribonomics Approaches to Identify RBPome in Plants and Other Eukaryotes: Current Progress and Future Prospects.International journal of molecular sciences · 2022Review
- Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Directed Evolution of Split APEX2 Peroxidase.ACS chemical biology · 2019Article
- Conserved function of the long noncoding RNA Blnc1 in brown adipocyte differentiation.Molecular metabolism · 2017Article
- Generation of Recombinant Polioviruses Harboring RNA Affinity Tags in the 5' and 3' Noncoding Regions of Genomic RNAs.Viruses · 2016Article
- Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.Nature methods · 2015Article
- A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation.Molecular cell · 2014Article
- Identification of sequence-structure RNA binding motifs for SELEX-derived aptamers.Bioinformatics (Oxford, England) · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
RNA affinity tags (aptamers) have emerged as useful tools for the isolation of RNAs and ribonucleoprotein complexes from cell extracts. The streptavidin binding RNA aptamer binds with high affinity and is quickly and cleanly eluted with biotin under mild conditions that retain intact complexes. We describe the use of the streptavidin binding aptamer as a tool for purification and discuss strategies towards the design and production of tagged RNAs with a focus on structured target RNAs. The aptamer site can be further exploited as a unique region for the hybridization of oligonucleotide probes and localization by fluorescent in situ hybridization (FISH). The aptamer insertion will allow the localization of a population of RNA species (such as mutants) to be viewed specifically, while in the presence of the wild type RNA. We describe the production of labeled oligonucleotide probes and the preparation of yeast cells for the localization of RNAs by FISH.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.