ArticleMethods in molecular biology (Clifton, N.J.)2011
Visualization of mRNA localization in Xenopus oocytes.
Article in Methods in molecular biology (Clifton, N.J.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Molecular Pathology of Sodium Channel Beta-Subunit Variants.Frontiers in pharmacology · 2021Article
- Methods for the analysis of early oogenesis in Zebrafish.Developmental biology · 2017Article
- Using in vivo imaging to measure RNA mobility in Xenopus laevis oocytes.Methods (San Diego, Calif.) · 2016Article
- Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.Molecular biology of the cell · 2015Article
- Essential elements for translation: the germline factor Vasa functions broadly in somatic cells.Development (Cambridge, England) · 2015Article
- Article
- Directional transport is mediated by a Dynein-dependent step in an RNA localization pathway.PLoS biology · 2013Article
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Authors and funding
2 authors.
Funding
Abstract
Visualization of in vivo mRNA localization provides a tool for understanding steps in the mechanism of transport. Here we detail a method of fluorescently labeling mRNA transcripts and microinjecting them into Xenopus laevis oocytes followed with imaging by confocal microscopy. This technique overcomes a significant hurdle of imaging RNA in the frog oocyte while providing a rapid method of visualizing mRNA localization in high resolution.
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Registered trials
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