ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
Effect of immunohistochemistry on molecular analysis of tissue samples: implications for microdissection technologies.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
- Novel Multimodal Imaging System for High-Resolution and High-Contrast Tissue Segmentation Based on Chemical Properties.Sensors (Basel, Switzerland) · 2025Article
- Interstitial deletion of the Apc locus in β-catenin-overexpressing cells is a signature of radiation-induced intestinal tumors in C3B6F1 ApcMin/+ mice†.Journal of radiation research · 2023Article
- Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2022Article
- Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection.Frontiers in cell and developmental biology · 2022Article
- Laser capture microdissection: techniques and applications in liver diseases.Hepatology international · 2019Review
- Laser capture microdissection protocol for gene expression analysis in the brain.Histochemistry and cell biology · 2017Article
- Optimized expression-based microdissection of formalin-fixed lung cancer tissue.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Article
- Approaches for targeted proteomics and its potential applications in neuroscience.Journal of biosciences · 2015Review
- The workflow from post-mortem human brain sampling to cell microdissection: a Brain Net Europe study.Journal of neural transmission (Vienna, Austria : 1996) · 2015Article
- Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection.Analytical chemistry · 2013Article
- Semi-nested real-time reverse transcription polymerase chain reaction methods for the successful quantitation of cytokeratin mRNA expression levels for the subtyping of non-small-cell lung carcinoma using paraffin-embedded and microdissected lung biopsy specimens.Acta histochemica et cytochemica · 2013Article
- Article
- Identification of unique expression signatures and therapeutic targets in esophageal squamous cell carcinoma.BMC research notes · 2012Article
- Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples.Acta cytologica · 2012Article
- Immunoguided microdissection techniques.Methods in molecular biology (Clifton, N.J.) · 2011Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Laser-based tissue microdissection is an important tool for the molecular evaluation of histological sections. The technology has continued to advance since its initial commercialization in the 1990s, with improvements in many aspects of the process. More recent developments are tailored toward an automated, operator-independent mode that relies on antibodies as targeting probes, such as immuno-laser capture microdissection or expression microdissection (xMD). Central to the utility of expression-based dissection techniques is the effect of the staining process on the biomolecules in histological sections. To investigate this issue, the authors analyzed DNA, RNA, and protein in immunostained, microdissected samples. DNA was the most robust molecule, exhibiting no significant change in quality after immunostaining but a variable 50% to 75% decrease in the total yield. In contrast, RNA in frozen and ethanol-fixed, paraffin-embedded samples was susceptible to hydrolysis and digestion by endogenous RNases during the initial steps of staining. Proteins from immunostained tissues were successfully analyzed by one-dimensional electrophoresis and mass spectrometry but were less amenable to solution phase assays. Overall, the results suggest investigators can use immunoguided microdissection methods for important analytic techniques; however, continued improvements in staining protocols and molecular extraction methods are key to further advancing the capability of these methods.
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