ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2011
Intrinsic bioenergetic properties and stress sensitivity of dopaminergic synaptosomes.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Dynamic changes in mitochondrial function in brain cortex synaptosomes during aging.Journal of bioenergetics and biomembranes · 2026Article
- Synaptosomes isolated from cryopreserved MND motor cortex reveal altered calcium handling and reduced complex IV-linked respiration.Frontiers in synaptic neuroscience · 2026Article
- Dopamine and Methamphetamine Differentially Affect Electron Transport Chain Complexes and Parkin in Rat Striatum: New Insight into Methamphetamine Neurotoxicity.International journal of molecular sciences · 2021Article
- Central nervous system-penetrating antiretrovirals impair energetic reserve in striatal nerve terminals.Journal of neurovirology · 2017Article
- Metabolic Dysfunction in Parkinson's Disease: Bioenergetics, Redox Homeostasis and Central Carbon Metabolism.Brain research bulletin · 2017Review
- Glucose, Lactate and Glutamine but not Glutamate Support Depolarization-Induced Increased Respiration in Isolated Nerve Terminals.Neurochemical research · 2017Article
- Article
- Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging.Neurochemical research · 2016Article
- Regulation of ATP13A2 via PHD2-HIF1α Signaling Is Critical for Cellular Iron Homeostasis: Implications for Parkinson's Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Article
- Comparison of the structure, function and autophagic maintenance of mitochondria in nigrostriatal and tuberoinfundibular dopamine neurons.Brain research · 2015Article
- The role of mitochondrially derived ATP in synaptic vesicle recycling.The Journal of biological chemistry · 2015Article
- Mitochondrial bioenergetics and neuronal survival modelled in primary neuronal culture and isolated nerve terminals.Journal of bioenergetics and biomembranes · 2015Review
- Synaptosomal lactate dehydrogenase isoenzyme composition is shifted toward aerobic forms in primate brain evolution.Brain, behavior and evolution · 2014Article
- Article
- SOD2 in mitochondrial dysfunction and neurodegeneration.Free radical biology & medicine · 2013Review
- Caspase 1 activation is protective against hepatocyte cell death by up-regulating beclin 1 protein and mitochondrial autophagy in the setting of redox stress.The Journal of biological chemistry · 2013Article
- Calcium, bioenergetics, and neuronal vulnerability in Parkinson's disease.The Journal of biological chemistry · 2013Review
- Article
- Neuronal vulnerability, pathogenesis, and Parkinson's disease.Movement disorders : official journal of the Movement Disorder Society · 2013Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Dopaminergic neurons of the substantia nigra pars compacta are defective in Parkinson's disease, but the specificity of this dysfunction is not understood. One hypothesis is that mitochondrial bioenergetic capacity is intrinsically lower in striatal dopaminergic presynaptic nerve varicosities, making them unusually susceptible to inhibition of electron transport by oxidative damage. To test this hypothesis, we separated isolated synaptosomes bearing dopamine transporters using immunomagnetic beads and compared their respiration with that of the residual nondopaminergic synaptosomes. As predicted, dopaminergic synaptosomes from striatum had lower respiratory rates. However, so did dopaminergic synaptosomes from cortex, indicating a lack of the predicted striatal specificity. We used fluorescent probes to analyze the bioenergetic competence of individual synaptosomes in the two fractions. The respiratory differences became nonsignificant when respiration rates were normalized to the number of respiration-competent synaptosomes, suggesting that differences reflected the quality of the different fractions. To circumvent damage induced by synaptosomal separation, we monitored membrane potentials in whole unseparated single synaptosomes using fluorescent imaging, and then identified the dopaminergic subpopulation using a fluorescent dopamine transporter substrate (ASP(+) [4-(4-diethylaminostyryl)-N-methylpyridinium iodide]). The capacity of dopaminergic and nondopaminergic synaptosomes to maintain plasma membrane and mitochondrial membrane potential under several stresses did not differ. In addition, this capacity did not decline in either subpopulation with age, a risk factor for Parkinson's disease. We conclude that the intrinsic bioenergetic capacities of dopaminergic and nondopaminergic presynaptic synaptosomes from mice do not differ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.