ArticleJournal of clinical microbiology2011
Replacing traditional diagnostics of fecal viral pathogens by a comprehensive panel of real-time PCRs.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.
- A systematic review and meta-analysis of the global prevalence of human enteric adenovirus infections.Journal of infection and public health · 2025Pooled it
- Article
- Co-infection and interaction of enteric pathogens in acute diarrhea among children under five years: a large-scale multicenter observational study from China.Infectious diseases of poverty · 2025Observational
- Snotwatch: an ecological analysis of the relationship between febrile seizures and respiratory virus activity.BMC pediatrics · 2022Article
- Outbreak of severe acute respiratory infection in Southern Province, Sri Lanka in 2018: a cross-sectional study.BMJ open · 2020Article
- Clinical and Imaging Findings of Neonatal Seizures Presenting as Diffuse Cerebral White Matter Abnormality on Diffusion-Weighted Imaging without any Structural or Metabolic Etiology.Taehan Yongsang Uihakhoe chi · 2020Article
- The Role of Oral Administration of Immunoglobulin in Managing Diarrheal Illness in Immunocompromised Children.Paediatric drugs · 2020Article
- Detection of norovirus infections in Denmark, 2011-2018.Epidemiology and infection · 2020Article
- Detection of different enteric viruses in children with diarrheal disease: evidence of the high frequency of mixed infections.Access microbiology · 2019Article
- Adenovirus associated with acute diarrhea: a case-control study.BMC infectious diseases · 2018Article
- Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia.BMC infectious diseases · 2018Article
- Detection of common diarrhea-causing pathogens in Northern Taiwan by multiplex polymerase chain reaction.Medicine · 2018Observational
- Coinfection with Enteric Pathogens in East African Children with Acute Gastroenteritis-Associations and Interpretations.The American journal of tropical medicine and hygiene · 2018Article
- Astrovirus Diagnostics.Viruses · 2017Review
- Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2016Article
- Distribution and Trends of Human Parainfluenza Viruses in Hospitalised Children.Indian journal of pediatrics · 2016Article
- Narrowing of the Diagnostic Gap of Acute Gastroenteritis in Children 0-6 Years of Age Using a Combination of Classical and Molecular Techniques, Delivers Challenges in Syndromic Approach Diagnostics.The Pediatric infectious disease journal · 2016Article
- Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses.Virology journal · 2016Article
- Aetiology of diarrhoeal disease and evaluation of viral-bacterial coinfection in children under 5 years old in China: a matched case-control study.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2016Article
- Fatal case of acute gastroenteritis with multiple viral coinfections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular DNA-based diagnostics are increasingly being used for diagnosis of viral infections. For enteric viruses, PCR assays have also been developed. The aims of this study were to compile and evaluate a comprehensive panel of PCR assays for diagnosis of viruses causing diarrheal disease and to evaluate its use in a largely pediatric population in a 750-bed university medical center. The PCR panel was designed to include assays for detection of adenovirus, astrovirus, enterovirus, norovirus, parechovirus, rotavirus, and sapovirus. The results of the PCR panel were evaluated in relation to conventional viral diagnostics consisting of viral culture and/or rotavirus and adenovirus rapid antigen tests on samples that were taken for routine diagnostics. Comparing conventional with PCR-based testing, the number of viruses detected increased dramatically from 25 to 106 when PCR assays were used. This increase was due mainly to detection of previously undetected viruses, i.e., astrovirus, norovirus, and sapovirus. In 24% of the samples, norovirus was detected. Also, the lower detection limit of PCR-based adenovirus, enterovirus, parechovirus, and rotavirus diagnostics further increased the detection rate. By focusing on samples from patients with complaints of gastroenteritis, detection of a causative agent was increased from 49% by conventional tests to 97% by molecular diagnostics. However, many samples containing low viral loads were found in patients with complaints other than intestinal complaints. In conclusion, the proposed comprehensive PCR panel with appropriate cutoff values can be used for sensitive, rapid, and clinically relevant diagnosis of gastrointestinal viruses.
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