ArticleJournal of virology2011
Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Subversion of selective autophagy for the biogenesis of tombusvirus replication organelles inhibits autophagy.PLoS pathogens · 2024Article
- Contribution of yeast models to virus research.Applied microbiology and biotechnology · 2021Review
- Dynamic interplay between the co-opted Fis1 mitochondrial fission protein and membrane contact site proteins in supporting tombusvirus replication.PLoS pathogens · 2021Article
- Reconstitution of an RNA Virus Replicase in Artificial Giant Unilamellar Vesicles Supports Full Replication and Provides Protection for the Double-Stranded RNA Replication Intermediate.Journal of virology · 2020Article
- Co-opting the fermentation pathway for tombusvirus replication: Compartmentalization of cellular metabolic pathways for rapid ATP generation.PLoS pathogens · 2019Article
- Perception of double-stranded RNA in plant antiviral immunity.Molecular plant pathology · 2019Review
- The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus.Journal of virology · 2018Article
- Cowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiae.PloS one · 2018Article
- Regulation of yeast ESCRT-III membrane scission activity by the Doa4 ubiquitin hydrolase.Molecular biology of the cell · 2017Article
- Distinct Roles for Intracellular and Extracellular Lipids in Hepatitis C Virus Infection.PloS one · 2016Article
- RNA virus replication depends on enrichment of phosphatidylethanolamine at replication sites in subcellular membranes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Host ESCRT proteins are required for bromovirus RNA replication compartment assembly and function.PLoS pathogens · 2015Article
- Inferring host gene subnetworks involved in viral replication.PLoS computational biology · 2014Article
- (+)RNA viruses rewire cellular pathways to build replication organelles.Current opinion in virology · 2012Review
- Ubiquitin and plant viruses, let's play together!Plant physiology · 2012Review
- Host co-factors of the retrovirus-like transposon Ty1.Mobile DNA · 2012Article
- Role of host reticulon proteins in rearranging membranes for positive-strand RNA virus replication.Current opinion in microbiology · 2012Review
- Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.Journal of virology · 2012Article
- Dengue virus infection perturbs lipid homeostasis in infected mosquito cells.PLoS pathogens · 2012Article
- West Nile virus replication requires fatty acid synthesis but is independent on phosphatidylinositol-4-phosphate lipids.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Like many positive-strand RNA viruses, brome mosaic virus (BMV) RNA replication occurs in membrane-invaginated vesicular compartments. BMV RNA replication compartments show parallels with membrane-enveloped, budding retrovirus virions, whose release depends on the cellular multivesicular body (MVB) sorting pathway. BMV RNA replication compartments are not released from their parent membranes, but might depend on MVB functions for membrane invagination. Prior results show that BMV RNA replication is severely inhibited by deletion of the crucial MVB gene DOA4 or BRO1. We report here that involvement of DOA4 and BRO1 in BMV RNA replication is not dependent on the MVB pathway's membrane-shaping functions but rather is due to their roles in recycling ubiquitin from MVB cargos. We show that deleting DOA4 or BRO1 inhibits the ubiquitination- and proteasome-dependent activation of homologous transcription factors Mga2p and Spt23p, which regulate many lipid metabolism genes, including the fatty acid desaturase gene OLE1, which is essential for BMV RNA replication. However, Mga2p processing and BMV RNA replication are restored by supplementing free ubiquitin, which is depleted in doa4Δ and bro1Δ cells. The results identify Mga2p and Spt23p processing and lipid regulation as sensitive targets of ubiquitin depletion and correctly predict multiple effects of modulating additional host genes RFU1, UBP6, and UFD3. Our results also show that BMV RNA replication depends on additional Mga2p-regulated genes likely involved in lipid metabolism beyond OLE1. Among other points, these findings show the potential for blocking viral RNA replication by modulating lipid synthesis at multiple levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.