ArticlePlant physiology2011
Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured BY-2 cells.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- In vitro reconstitution of COPII vesicles from Arabidopsis thaliana suspension-cultured cells.Nature protocols · 2023Review
- Article
- Armadillo-repeat kinesin1 interacts with Arabidopsis atlastin RHD3 to move ER with plus-end of microtubules.Nature communications · 2020Article
- NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.Journal of experimental botany · 2017Article
- Myosin-driven transport network in plants is functionally robust and distinctive.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Plant ER geometry and dynamics: biophysical and cytoskeletal control during growth and biotic response.Protoplasma · 2017Review
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
- Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.Plant physiology · 2016Article
- Protein Bodies in Leaves Exchange Contents through the Endoplasmic Reticulum.Frontiers in plant science · 2016Article
- Interactions between plant endomembrane systems and the actin cytoskeleton.Frontiers in plant science · 2015Review
- Microtubules contribute to tubule elongation and anchoring of endoplasmic reticulum, resulting in high network complexity in Arabidopsis.Plant physiology · 2014Article
- Artificially-induced organelles are optimal targets for optical trapping experiments in living cells.Biomedical optics express · 2014Article
- Genome-wide identification, splicing, and expression analysis of the myosin gene family in maize (Zea mays).Journal of experimental botany · 2014Article
- ER network dynamics are differentially controlled by myosins XI-K, XI-C, XI-E, XI-I, XI-1, and XI-2.Frontiers in plant science · 2014Article
- Involvement of the sieve element cytoskeleton in electrical responses to cold shocks.Plant physiology · 2013Article
- Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI.The Journal of biological chemistry · 2012Article
- Opaque1 encodes a myosin XI motor protein that is required for endoplasmic reticulum motility and protein body formation in maize endosperm.The Plant cell · 2012Article
- Plant-Specific Myosin XI, a Molecular Perspective.Frontiers in plant science · 2012Article
- Are kinesins required for organelle trafficking in plant cells?Frontiers in plant science · 2012Article
- Myosin XIK is a major player in cytoplasm dynamics and is regulated by two amino acids in its tail.Journal of experimental botany · 2012Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reticular network of the endoplasmic reticulum (ER) consists of tubular and lamellar elements and is arranged in the cortical region of plant cells. This network constantly shows shape change and remodeling motion. Tubular ER structures were formed when GTP was added to the ER vesicles isolated from tobacco (Nicotiana tabacum) cultured BY-2 cells expressing ER-localized green fluorescent protein. The hydrolysis of GTP during ER tubule formation was higher than that under conditions in which ER tubule formation was not induced. Furthermore, a shearing force, such as the flow of liquid, was needed for the elongation/extension of the ER tubule. The shearing force was assumed to correspond to the force generated by the actomyosin system in vivo. To confirm this hypothesis, the S12 fraction was prepared, which contained both cytosol and microsome fractions, including two classes of myosins, XI (175-kD myosin) and VIII (BY-2 myosin VIII-1), and ER-localized green fluorescent protein vesicles. The ER tubules and their mesh-like structures were arranged in the S12 fraction efficiently by the addition of ATP, GTP, and exogenous filamentous actin. The tubule formation was significantly inhibited by the depletion of 175-kD myosin from the S12 fraction but not BY-2 myosin VIII-1. Furthermore, a recombinant carboxyl-terminal tail region of 175-kD myosin also suppressed ER tubule formation. The tips of tubules moved along filamentous actin during tubule elongation. These results indicated that the motive force generated by the actomyosin system contributes to the formation of ER tubules, suggesting that myosin XI is responsible not only for the transport of ER in cytoplasm but also for the reticular organization of cortical ER.
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