ArticleDevelopment (Cambridge, England)2011
The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 46 citations in OpenAlex.
- Cell-type-resolved RNA-seq reveals molecular signatures of sexual experience inNAR genomics and bioinformatics · 2026Article
- TENT-5 polyadenylates and regulates male-specific transcripts in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2026Article
- Evolution of the reproductive interactome and the origins of postmating-prezygotic reproductive divergence.BMC biology · 2026Article
- Terminal nucleotidyltransferaseRNA (New York, N.Y.) · 2025Article
- Testis- and ovary-expressed polo-like kinase transcripts and gene duplications affect male fertility when expressed in the Drosophila melanogaster germline.G3 (Bethesda, Md.) · 2025Article
- TENT5-mediated polyadenylation of mRNAs encoding secreted proteins is essential for gametogenesis in mice.Nature communications · 2024Article
- A postmeiotically bifurcated roadmap of honeybee spermatogenesis marked by phylogenetically restricted genes.PLoS genetics · 2023Article
- The Battle for Survival: The Role of RNA Non-Canonical Tails in the Virus-Host Interaction.Metabolites · 2023Review
- Cytoplasmic Polyadenylation Is an Ancestral Hallmark of Early Development in Animals.Molecular biology and evolution · 2023Article
- Gld2 activity and RNA specificity is dynamically regulated by phosphorylation and interaction with QKI-7.RNA biology · 2021Article
- Functions and mechanisms of RNA tailing by metazoan terminal nucleotidyltransferases.Wiley interdisciplinary reviews. RNA · 2021Review
- Structures of mammalian GLD-2 proteins reveal molecular basis of their functional diversity in mRNA and microRNA processing.Nucleic acids research · 2020Article
- A tale of non-canonical tails: gene regulation by post-transcriptional RNA tailing.Nature reviews. Molecular cell biology · 2020Review
- Terminal nucleotidyl transferases (TENTs) in mammalian RNA metabolism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- 5' and 3' modifications controlling RNA degradation: from safeguards to executioners.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Genes Relocated Between Drosophila Chromosome Arms Evolve Under Relaxed Selective Constraints Relative to Non-Relocated Genes.Journal of molecular evolution · 2018Article
- Polyadenylation is the key aspect of GLD-2 function inRNA (New York, N.Y.) · 2017Article
- The Goddard and Saturn Genes Are Essential for Drosophila Male Fertility and May Have Arisen De Novo.Molecular biology and evolution · 2017Article
- Structural basis for the antagonistic roles of RNP-8 and GLD-3 in GLD-2 poly(A)-polymerase activity.RNA (New York, N.Y.) · 2016Article
- Nucleotide specificity of the human terminal nucleotidyltransferase Gld2 (TUT2).RNA (New York, N.Y.) · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The DNA of a developing sperm is normally inaccessible for transcription for part of spermatogenesis in many animals. In Drosophila melanogaster, many transcripts needed for late spermatid differentiation are synthesized in pre-meiotic spermatocytes, but are not translated until later stages. Thus, post-transcriptional control mechanisms are required to decouple transcription and translation during spermatogenesis. In the female germline, developing germ cells accomplish similar decoupling through poly(A) tail alterations to ensure that dormant transcripts are not prematurely translated: a transcript with a short poly(A) tail will remain untranslated, whereas elongating the poly(A) tail permits protein production. In Drosophila, the ovary-expressed cytoplasmic poly(A) polymerase WISPY is responsible for stage-specific poly(A) tail extension in the female germline. Here, we examine the possibility that a recently derived testis-expressed WISPY paralog, GLD2, plays a similar role in the Drosophila male germline. We show that knockdown of Gld2 transcripts causes male sterility, as GLD2-deficient males do not produce mature sperm. Spermatogenesis up to and including meiosis appears normal in the absence of GLD2, but post-meiotic spermatid development rapidly becomes abnormal. Nuclear bundling and F-actin assembly are defective in GLD2 knockdown testes and nuclei fail to undergo chromatin reorganization in elongated spermatids. GLD2 also affects the incorporation of protamines and the stability of dynamin and transition protein transcripts. Our results indicate that GLD2 is an important regulator of late spermatogenesis and is the first example of a Gld-2 family member that plays a significant role specifically in male gametogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.