ArticleACS chemical biology2011
Metabolic labeling of fucosylated glycans in developing zebrafish.
Article in ACS chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Enzyme-Linked Cycloaddition Assay (ELCA) for Rapid, Ultra-Sensitive Monitoring of Secreted Sialoglycoproteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comparison of human intestinal enteroid and zebrafish larva models for replication of human norovirus.Applied and environmental microbiology · 2026Article
- Investigation Into the Dynamics of the Cupula in the Vestibular Organ of Adult Zebrafish Using Metabolic Glycoengineering.Angewandte Chemie (International ed. in English) · 2026Article
- Exploring Metal-Free Click Reactions: New Frontiers in Glycochemistry and Bioconjugation.Bioconjugate chemistry · 2025Review
- Review
- Ammoxidation of Unprotected Glycosides: A One-Pot Conversion of Alcohols to Nitriles.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Bio-orthogonal Glycan Imaging of Cultured Cells and Whole AnimalACS central science · 2025Article
- Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose.Glycobiology · 2025Article
- Quantitative analysis of fucosylated glycoproteins by immobilized lectin-affinity fluorescent labeling.RSC advances · 2023Article
- Article
- Therapeutic Monosaccharides: Looking Back, Moving Forward.Biochemistry · 2020Review
- Quantitative capillary zone electrophoresis-mass spectrometry reveals the N-glycome developmental plan during vertebrate embryogenesis.Molecular omics · 2020Article
- Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development.Molecules (Basel, Switzerland) · 2020Article
- Direct Visualization of Live Zebrafish Glycans via Single-Step Metabolic Labeling with Fluorophore-Tagged Nucleotide Sugars.Angewandte Chemie (International ed. in English) · 2019Review
- Pathogenic Variants in Fucokinase Cause a Congenital Disorder of Glycosylation.American journal of human genetics · 2018Article
- Systems glycomics of adult zebrafish identifies organ-specific sialylation and glycosylation patterns.Nature communications · 2018Article
- Asking more from metabolic oligosaccharide engineering.Chemical science · 2018Review
- Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation.Chemical science · 2018Article
- Practical Considerations, Challenges, and Limitations of Bioconjugation via Azide-Alkyne Cycloaddition.Bioconjugate chemistry · 2018Review
- Mechanistic Investigation and Multiplexing of Liposome-Assisted Metabolic Glycan Labeling.Journal of the American Chemical Society · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Many developmental processes depend on proper fucosylation, but this post-translational modification is difficult to monitor in vivo. Here we applied a chemical reporter strategy to visualize fucosylated glycans in developing zebrafish. Using azide-derivatized analogues of fucose, we metabolically labeled cell-surface glycans and then detected the incorporated azides via copper-free click chemistry with a difluorinated cyclooctyne probe. We found that the fucose salvage pathway enzymes are expressed during zebrafish embryogenesis but that they process the azide-modified substrates inefficiently. We were able to bypass the salvage pathway by using an azide-functionalized analogue of GDP-fucose. This nucleotide sugar was readily accepted by fucosyltransferases and provided robust cell-surface labeling of fucosylated glycans, as determined by flow cytometry and confocal microscopy analysis. We used this technique to image fucosylated glycans in the enveloping layer of zebrafish embryos during the first 5 days of development. This work provides a method to study the biosynthesis of fucosylated glycans in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.