ArticleStem cells (Dayton, Ohio)2011
MyoD gene suppression by Oct4 is required for reprogramming in myoblasts to produce induced pluripotent stem cells.
Article in Stem cells (Dayton, Ohio), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
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- In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche.Nature communications · 2021Article
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- Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues.World journal of stem cells · 2019Article
- Inhibition of microRNA-92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy-related phenotype in mdx mice.Muscle & nerve · 2019Article
- Non-viral, Tumor-free Induction of Transient Cell Reprogramming in Mouse Skeletal Muscle to Enhance Tissue Regeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.Scientific reports · 2018Article
- Skeletal Muscle Cell Induction from Pluripotent Stem Cells.Stem cells international · 2017Review
- Induced pluripotent stem cells in Alzheimer's disease: applications for disease modeling and cell-replacement therapy.Molecular neurodegeneration · 2016Review
- Creating Interactions between Tissue-Engineered Skeletal Muscle and the Peripheral Nervous System.Cells, tissues, organs · 2016Article
- Pregnancy-induced amelioration of muscular dystrophy phenotype in mdx mice via muscle membrane stabilization effect of glucocorticoid.PloS one · 2015Article
- Competence of in vitro cultured mouse embryonic stem cells for myogenic differentiation and fusion with myoblasts.Stem cells and development · 2014Article
- Grand challenges in the field of stem cell research.Frontiers in cell and developmental biology · 2014Article
- Transcription factors interfering with dedifferentiation induce cell type-specific transcriptional profiles.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres.Cell reports · 2013Article
- Intrinsic ability of adult stem cell in skeletal muscle: an effective and replenishable resource to the establishment of pluripotent stem cells.Stem cells international · 2013Article
- Article
- Skeletal Muscle-derived Hematopoietic Stem Cells: Muscular Dystrophy Therapy by Bone Marrow Transplantation.Journal of stem cell research & therapy · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Expression of the four transcription factors, that is, Oct4, Sox2, cMyc, and Klf4 has been shown to generate induced pluripotent stem cells (iPSCs) from many types of specialized differentiated somatic cells. It remains unclear, however, whether fully committed skeletal muscle progenitor cells (myoblasts) have the potency to undergo reprogramming to develop iPSCs in line with previously reported cases. To test this, we have isolated genetically marked myoblasts derived from satellite cell of adult mouse muscles using the Cre-loxP system (Pax7-CreER:R26R and Myf5-Cre:R26R). On infection with retroviral vectors expressing the four factors, these myoblasts gave rise to myogenic lineage tracer lacZ-positive embryonic stem cell (ESC)-like colonies. These cells expressed ESC-specific genes and were competent to differentiate into all three germ layers and germ cells, indicating the successful generation of myoblast-derived iPSCs. Continuous expression of the MyoD gene, a master transcription factor for skeletal muscle specification, inhibited this reprogramming process in myoblasts. In contrast, reprogramming myoblasts isolated from mice lacking the MyoD gene led to an increase in reprogramming efficiency. Our data also indicated that Oct4 acts as a transcriptional suppressor of MyoD gene expression through its interaction with the upstream enhancer region. Taken together, these results indicate that suppression of MyoD gene expression by Oct4 is required for the initial reprogramming step in the development of iPSCs from myoblasts. This data suggests that the skeletal muscle system provides a well-defined differentiation model to further elaborate on the effects of iPSC reprogramming in somatic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.