ArticleClinical orthopaedics and related research2011
Cartilage matrix formation by bovine mesenchymal stem cells in three-dimensional culture is age-dependent.
Article in Clinical orthopaedics and related research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.Annals of biomedical engineering · 2026Article
- Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging.bioRxiv : the preprint server for biology · 2025Article
- Precision repair of zone-specific meniscal injuries using a tunable extracellular matrix-based hydrogel system.Bioactive materials · 2025Article
- Donor age and breed determine mesenchymal stromal cell characteristics.Stem cell research & therapy · 2025Article
- An Update on Applications of Cattle Mesenchymal Stromal Cells.Animals : an open access journal from MDPI · 2022Review
- Accelerated Discovery of the Polymer Blends for Cartilage Repair through Data-Mining Tools and Machine-Learning Algorithm.Polymers · 2022Article
- A Transgenic Rat for Noninvasive Assessment of ChondrogenesisCartilage · 2021Article
- Fabrication and maturation of integrated biphasic anatomic mesenchymal stromal cell-laden composite scaffolds for osteochondral repair and joint resurfacing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells.Veterinary world · 2020Article
- Determinants of stem cell lineage differentiation toward chondrogenesis versus adipogenesis.Cellular and molecular life sciences : CMLS · 2019Review
- Extracellular vesicles mediate improved functional outcomes in engineered cartilage produced from MSC/chondrocyte cocultures.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Applications of mesenchymal stem cell technology in bovine species.Stem cell research & therapy · 2019Review
- Donor Variation and Optimization of Human Mesenchymal Stem Cell Chondrogenesis in Hyaluronic Acid.Tissue engineering. Part A · 2018Article
- Review
- Hyaluronic Acid (HA) Scaffolds and Multipotent Stromal Cells (MSCs) in Regenerative Medicine.Stem cell reviews and reports · 2016Review
- Chemical and Physical Approaches to Extend the Replicative and Differentiation Potential of Stem Cells.Stem cell reviews and reports · 2016Review
- Anatomic Mesenchymal Stem Cell-Based Engineered Cartilage Constructs for Biologic Total Joint Replacement.Tissue engineering. Part A · 2016Article
- Changes in Chondrogenic Progenitor Populations Associated with Aging and Osteoarthritis.Cartilage · 2015Article
- Delivering heparin-binding insulin-like growth factor 1 with self-assembling peptide hydrogels.Tissue engineering. Part A · 2015Article
- Senescence suppressors: their practical importance in replicative lifespan extension in stem cells.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCartilage degeneration is common in the aged, and aged chondrocytes are inferior to juvenile chondrocytes in producing cartilage-specific extracellular matrix. Mesenchymal stem cells (MSCs) are an alternative cell type that can differentiate toward the chondrocyte phenotype. Aging may influence MSC chondrogenesis but remains less well studied, particularly in the bovine system. QUESTIONS/PURPOSES: The objectives of this study were (1) to confirm age-related changes in bovine articular cartilage, establish how age affects chondrogenesis in cultured pellets for (2) chondrocytes and (3) MSCs, and (4) determine age-related changes in the biochemical and biomechanical development of clinically relevant MSC-seeded hydrogels.
methodsNative bovine articular cartilage from fetal (n = 3 donors), juvenile (n = 3 donors), and adult (n = 3 donors) animals was analyzed for mechanical and biochemical properties (n = 3-5 per donor). Chondrocyte and MSC pellets (n = 3 donors per age) were cultured for 6 weeks before analysis of biochemical content (n = 3 per donor). Bone marrow-derived MSCs of each age were also cultured within hyaluronic acid hydrogels for 3 weeks and analyzed for matrix deposition and mechanical properties (n = 4 per age).
resultsArticular cartilage mechanical properties and collagen content increased with age. We observed robust matrix accumulation in three-dimensional pellet culture by fetal chondrocytes with diminished collagen-forming capacity in adult chondrocytes. Chondrogenic induction of MSCs was greater in fetal and juvenile cell pellets. Likewise, fetal and juvenile MSCs in hydrogels imparted greater matrix and mechanical properties.
conclusionsDonor age and biochemical microenvironment were major determinants of both bovine chondrocyte and MSC functional capacity. CLINICAL RELEVANCE: In vitro model systems should be evaluated in the context of age-related changes and should be benchmarked against human MSC data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.