ArticleCellular and molecular neurobiology2011
In vitro differentiation of mouse embryonic stem cells into neurons of the dorsal forebrain.
Article in Cellular and molecular neurobiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Specification of murine ground state pluripotent stem cells to regional neuronal populations.Scientific reports · 2017Article
- Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke.Progress in neurobiology · 2017Review
- Longitudinal RNA sequencing of the deep transcriptome during neurogenesis of cortical glutamatergic neurons from murine ESCs.F1000Research · 2013Article
- Tracing synaptic connectivity onto embryonic stem cell-derived neurons.Stem cells (Dayton, Ohio) · 2012Article
- Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons.Frontiers in cellular neuroscience · 2012Article
- Genome-wide in silico identification of new conserved and functional retinoic acid receptor response elements (direct repeats separated by 5 bp).The Journal of biological chemistry · 2011Article
- Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Neural Commitment of Embryonic Stem Cells through the Formation of Embryoid Bodies (EBs).The Malaysian journal of medical sciences : MJMSArticle
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pluripotent embryonic stem cells (ESCs) are able to differentiate into all cell types in the organism including cortical neurons. To follow the dynamic generation of progenitors of the dorsal forebrain in vitro, we generated ESCs from D6-GFP mice in which GFP marks neocortical progenitors and neurons after embryonic day (E) 10.5. We used several cell culture protocols for differentiation of ESCs into progenitors and neurons of the dorsal forebrain. In cell culture, GFP-positive cells were induced under differentiation conditions in quickly formed embryoid bodies (qEBs) after 10-12 day incubation. Activation of Wnt signaling during ESC differentiation further stimulated generation of D6-GFP-positive cortical cells. In contrast, differentiation protocols using normal embryoid bodies (nEBs) yielded only a few D6-GFP-positive cells. Gene expression analysis revealed that multiple components of the canonical Wnt signaling pathway were expressed during the development of embryoid bodies. As shown by immunohistochemistry and quantitative qRT-PCR, D6-GFP-positive cells from qEBs expressed genes that are characteristic for the dorsal forebrain such as Pax6, Dach1, Tbr1, Tbr2, or Sox5. qEBs culture allowed the formation of a D6-GFP positive pseudo-polarized neuroepithelium with the characteristic presence of N-cadherin at the apical pole resembling the structure of the developing neocortex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.