Evidence map›Paper›PMID 21424396›Full record

ArticleDiabetologia2011

Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes.

S Larsen, N Stride, M Hey-Mogensen, C N Hansen, J L Andersen, S Madsbad, D Worm, J W Helge, F Dela

3 registry-linked trialsOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Diabetologia, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 3 registered trials, which are not on this map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02255682 phase4completedstarted 2015, after this paper: background citation

Living With Statins - The Impact of Cholesterol Lowering Drugs on Health, Lifestyle and Well-being

Ran2015Enrolled35Registered outcomes5Posted comparisons0ConditionsCardiovascular Disease, Diabetes MellitusArmsQ10, simvastatin
Open the trial in the graph
NCT02796378 phase4unknown statusstarted 2016, after this paper: background citation

Living With Statins - The Impact of Cholesterol Lowering Drugs on Health, Lifestyle and Well-being

Ran2016Enrolled30Registered outcomes5Posted comparisons0ConditionsCardiovascular Disease, Diabetes MellitusArmsTraining+Simvastatin-placebo+Q10-placebo, Training+Simvastatin+Q10, Training+Simvastatin+Q10-placebo
Open the trial in the graph
NCT02250677 completednot on this mapstarted 2014, after this paper: background citation

LIFESTAT - Living With Statins, a Cross Sectional Study on the Impact of Cholesterol Lowering Drugs on Health, Lifestyle and Well-being

TypeobservationalSponsorUniversity of CopenhagenRan2014 to 2016Enrolled75ConditionsCardiovascular Disease, Diabetes Mellitus
3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 70 citations in OpenAlex.

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  7. Normalization of maternal adiponectin in obese pregnant mice prevents programming of impaired glucose metabolism in adult offspring.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022
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  10. Targeting Mitochondria in Diabetes.International journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

S LarsenCenter for Healthy Aging, Department of Biomedical Sciences, Faculty of Health Sciences, Copenhagen University, Blegdamsvej 3b, 2200 Copenhagen N, Denmark. stelar@sund.ku.dk
N Stride
M Hey-Mogensen
C N Hansen
J L Andersen
S Madsbad
D Worm
J W Helge
F Dela
University of Copenhagen · DKBispebjerg Hospital · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aims/hypothesisMitochondrial respiration has been linked to insulin resistance. We studied mitochondrial respiratory capacity and substrate sensitivity in patients with type 2 diabetes (patients), and obese and lean control participants.

methodsMitochondrial respiration was measured in permeabilised muscle fibres by respirometry. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl-carnitine). Myosin heavy chain (MHC) composition, antioxidant capacity (manganese superoxide dismutase [MnSOD]), citrate synthase activity and maximal oxygen uptake (VO2) were also determined. Insulin sensitivity was determined with the isoglycaemic-hyperinsulinaemic clamp technique.

resultsInsulin sensitivity was different (p < 0.05) between the groups (patients<obese controls<lean controls). MnSOD was lower in patients than in lean controls. MHC I content was lowest in patients (37 ± 11% [mean ± SE] vs 53 ± 6% and 56 ± 4%) vs obese controls and lean controls, respectively. VO2 was highest in lean controls (40 ± 3 ml min(-1) kg(-1) [mean ± SE]) compared with patients (25 ± 2) and obese controls (27 ± 2). Mitochondrial content (citrate synthase) was higher (p < 0.05) in lean controls than in patients and obese controls. When normalised for mitochondrial content by citrate synthase, mitochondrial respiratory capacity was similar in all groups. However, the half maximal substrate concentration (C(50)) for complex I was significantly lower (p = 0.03) in patients (1.1 ± 0.2 mmol/l [mean ± SE]) than in obese (2.0 ± 0.3) and lean (1.8 ± 0.3) controls. Likewise, C(50) for complex II was lower (p = 0.02) in patients (3.5 ± 0.2 mmol/l [mean ± SE]) than in obese controls (4.1 ± 0.2), but did not differ from that in lean controls (3.8 ± 0.4). Substrate sensitivity for octanoyl-carnitine did not differ between groups. CONCLUSIONS/

interpretationIncreased mitochondrial substrate sensitivity is seen in skeletal muscle from type 2 diabetic patients and is confined to non-lipid substrates. Respiratory capacity per mitochondrion is not decreased with insulin resistance.

Indexed as

CarnitineCase-Control StudiesCell RespirationCitrate (si)-SynthaseDiabetes Mellitus, Type 2Glutamic AcidHumansInsulin ResistanceMaleMiddle AgedMitochondria, MuscleMuscle, SkeletalMyosin Heavy ChainsObesitySubstrate SpecificitySuccinic AcidCarnitineCitrate (si)-SynthaseGlutamic AcidMyosin Heavy ChainsoctanoylcarnitineSuccinic AcidSuperoxide Dismutase

Identifiers

PMID21424396
OpenAlexW2053265995

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.