ArticlePharmaceutical research2011
Non-viral delivery of inductive and suppressive genes to adipose-derived stem cells for osteogenic differentiation.
Article in Pharmaceutical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Enhanced Osteogenic Potential ofACS biomaterials science & engineering · 2023Article
- Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering.Journal of biological engineering · 2023Review
- Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation.Advanced healthcare materials · 2023Article
- Application of Cytokines of the Bone Morphogenetic Protein (BMP) Family in Spinal Fusion - Effects on the Bone, Intervertebral Disc and Mesenchymal Stromal Cells.Current stem cell research & therapy · 2019Review
- Comparison of osteogenic differentiation induced byInternational journal of nanomedicine · 2019Article
- Down-regulation of Noggin and miR-138 coordinately promote osteogenesis of mesenchymal stem cells.Journal of molecular histology · 2017Article
- Photocrosslinkable, biodegradable hydrogels with controlled cell adhesivity for prolonged siRNA delivery to hMSCs to enhance their osteogenic differentiation.Journal of materials chemistry. B · 2017Article
- Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels.Nanomedicine (London, England) · 2016Article
- Spatial control of cell gene expression by siRNA gradients in biodegradable hydrogels.Advanced healthcare materials · 2015Article
- Gene delivery of osteoinductive signals to a human fetal osteoblast cell line induces cell death in a dose-dependent manner.Drug delivery and translational research · 2015Article
- Nanocarrier mediated delivery of siRNA/miRNA in combination with chemotherapeutic agents for cancer therapy: current progress and advances.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Adipose-derived stem cells and BMP-2 delivery in chitosan-based 3D constructs to enhance bone regeneration in a rat mandibular defect model.Tissue engineering. Part A · 2014Article
- Sustained localized presentation of RNA interfering molecules from in situ forming hydrogels to guide stem cell osteogenic differentiation.Biomaterials · 2014Article
- The roles of bone morphogenetic proteins and their signaling in the osteogenesis of adipose-derived stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Developing siRNA therapies to address osteoporosis.Therapeutic delivery · 2013Review
- GNAS1 and PHD2 short-interfering RNA support bone regeneration in vitro and in an in vivo sheep model.Clinical orthopaedics and related research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo assess the effects of co-delivering osteoinductive DNA and/or small interfering RNA in directing the osteogenic differentiation of human adipose-derived stem cells (hADSCs) using a combinatorial, non-viral gene delivery approach.
methodshADSCs were transfected using combinations of the following genes: BMP2, siGNAS and siNoggin using poly(β-amino esters) or lipid-like molecules. A total of 15 groups were evaluated by varying DNA doses, timing of treatment, and combinations of signals. All groups were cultured in osteogenic medium for up to 37 days, and outcomes were measured using gene expression, biochemical assays, and histology.
resultsBiomaterials-mediated gene delivery led to a dose-dependent up-regulation of BMP2 and significant gene silencing of GNAS and Noggin in hADSCs. BMP2 alone slightly up-regulates osteogenic marker expression in hADSCs. In contrast, co-delivery of BMP2 and siGNAS or siNoggin significantly accelerates the hADSC differentiation towards osteogenic differentiation, with marked increase in bone marker expression and mineralization.
conclusionsWe report a combinatorial platform for identifying synergistic interactions among multiple genetic signals associated with osteogenic differentiation of hADSCs. Our results suggest that inductive or suppressive genetic switches interact in a complex manner, and highlight the promise of combinatorial approaches towards rapidly identifying optimal signals for promoting desired stem cell differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.