ArticlePloS one2011
Tandem quadruplication of HMA4 in the zinc (Zn) and cadmium (Cd) hyperaccumulator Noccaea caerulescens.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 122 citations in OpenAlex.
- Cis-regulatory elements co-opting core circadian clock regulator CCA1 underlie enhanced expression of HMA4 for metal hyperaccumulation in Arabidopsis halleri.Plant communications · 2025Article
- Towards Understanding the Factors Shaping the Composition and Function of theInternational journal of molecular sciences · 2025Review
- Article
- Multimodal synchrotron X-ray fluorescence imaging reveals elemental distribution in seeds and seedlings of the Zn-Cd-Ni hyperaccumulator Noccaea caerulescens.Metallomics : integrated biometal science · 2022Article
- Low-molecular-weight ligands in plants: role in metal homeostasis and hyperaccumulation.Photosynthesis research · 2021Review
- Comparative and Systematic Omics Revealed Low Cd Accumulation of PotatoInternational journal of molecular sciences · 2021Article
- Arabidopsis thaliana zinc accumulation in leaf trichomes is correlated with zinc concentration in leaves.Scientific reports · 2021Article
- Transcriptional Regulation of Genes Involved in Zinc Uptake, Sequestration and Redistribution Following Foliar Zinc Application toPlants (Basel, Switzerland) · 2021Article
- Transcriptome Response to Cadmium Exposure in Barley (Frontiers in plant science · 2021Article
- Exogenous abscisic acid (ABA) promotes cadmium (Cd) accumulation in Sedum alfredii Hance by regulating the expression of Cd stress response genes.Environmental science and pollution research international · 2020Article
- Elevated Expression of Vacuolar Nickel Transporter GeneFrontiers in plant science · 2020Article
- Phytoremediation of Heavy Metal Pollution: A Bibliometric and Scientometric Analysis from 1989 to 2018.International journal of environmental research and public health · 2019Article
- Comparative expression analysis of heavy metal ATPase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces.Plant diversity · 2019Article
- Towards an Understanding of the Molecular Basis of Nickel Hyperaccumulation in Plants.Plants (Basel, Switzerland) · 2019Review
- Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.Journal of experimental botany · 2019Article
- Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs.BMC genomics · 2018Article
- De novo transcriptome assemblies of four accessions of the metal hyperaccumulator plant Noccaea caerulescens.Scientific data · 2017Article
- Article
- Between-species differences in gene copy number are enriched among functions critical for adaptive evolution in Arabidopsis halleri.BMC genomics · 2016Article
- Conserved but Attenuated Parental Gene Expression in Allopolyploids: Constitutive Zinc Hyperaccumulation in the Allotetraploid Arabidopsis kamchatica.Molecular biology and evolution · 2016Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Zinc (Zn) and cadmium (Cd) hyperaccumulation may have evolved twice in the Brassicaceae, in Arabidopsis halleri and in the Noccaea genus. Tandem gene duplication and deregulated expression of the Zn transporter, HMA4, has previously been linked to Zn/Cd hyperaccumulation in A. halleri. Here, we tested the hypothesis that tandem duplication and deregulation of HMA4 expression also occurs in Noccaea.A Noccaea caerulescens genomic library was generated, containing 36,864 fosmid pCC1FOS™ clones with insert sizes ∼20-40 kbp, and screened with a PCR-generated HMA4 genomic probe. Gene copy number within the genome was estimated through DNA fingerprinting and pooled fosmid pyrosequencing. Gene copy numbers within individual clones was determined by PCR analyses with novel locus specific primers. Entire fosmids were then sequenced individually and reads equivalent to 20-fold coverage were assembled to generate complete whole contigs.Four tandem HMA4 repeats were identified in a contiguous sequence of 101,480 bp based on sequence overlap identities. These were flanked by regions syntenous with up and downstream regions of AtHMA4 in Arabidopsis thaliana. Promoter-reporter β-glucuronidase (GUS) fusion analysis of a NcHMA4 in A. thaliana revealed deregulated expression in roots and shoots, analogous to AhHMA4 promoters, but distinct from AtHMA4 expression which localised to the root vascular tissue.This remarkable consistency in tandem duplication and deregulated expression of metal transport genes between N. caerulescens and A. halleri, which last shared a common ancestor >40 mya, provides intriguing evidence that parallel evolutionary pathways may underlie Zn/Cd hyperaccumulation in Brassicaceae.
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