ArticlePloS one2011
Substoichiometrically different mitotypes coexist in mitochondrial genomes of Brassica napus L.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 164 citations in OpenAlex.
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- Worldwide Population Genomics Reveal Long-Term Stability of the Mitochondrial Genome Architecture in a Keystone Marine Plant.Genome biology and evolution · 2023Article
- Characterization of the complete mitochondrial genome of Cryptotermes domesticus (Blattodea: Kalotermitidae): Genome description and phylogenetic implications.Archives of insect biochemistry and physiology · 2023Article
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- Comparative analysis of mitochondrial genomes of two alpine medicinal plants of Gentiana (Gentianaceae).PloS one · 2023Article
- Comparative analysis of mitochondrial genomes provides insights into the mechanisms underlying an S-type cytoplasmic male sterility (CMS) system in wheat (Triticum aestivum L.).Functional & integrative genomics · 2022Article
- Comparative analysis of mitochondrial genomes of maize CMS-S subtypes provides new insights into male sterility stability.BMC plant biology · 2022Article
- Discovery of a novel mitochondrial DNA molecule associated with tetrad pollen sterility in potato.BMC plant biology · 2022Article
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- Comparative analysis of the complete mitochondrial genomes of four cordyceps fungi.Ecology and evolution · 2022Article
- Rapid and Synchronous Breeding of Cytoplasmic Male Sterile and Maintainer Line Through Mitochondrial DNA Rearrangement Using Doubled Haploid Inducer inFrontiers in plant science · 2022Article
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- Analysis of mitochondrial recombination in the male sterile Brassica juncea cybrid Og1 and identification of the molecular basis of fertility reversion.Plant molecular biology · 2021Article
- The first complete mitochondrial genome of eggplant (Mitochondrial DNA. Part B, Resources · 2021Article
- Comparative analysis of mitochondrial genomes of soybean cytoplasmic male-sterile lines and their maintainer lines.Functional & integrative genomics · 2021Article
6 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic male sterility (CMS) has been identified in numerous plant species. Brassica napus CMS plants, such as Polima (pol), MI, and Shaan 2A, have been identified independently by different researchers with different materials in conventional breeding processes. How this kind of CMS emerges is unclear. Here, we report the mitochondrial genome sequence of the prevalent mitotype in the most widely used pol-CMS line, which has a length of 223,412 bp and encodes 34 proteins, 3 ribosomal RNAs, and 18 tRNAs, including two near identical copies of trnH. Of these 55 genes, 48 were found to be identical to their equivalents in the "nap" cytoplasm. The nap mitotype carries only one copy of trnH, and the sequences of five of the six remaining genes are highly similar to their equivalents in the pol mitotype. Forty-four open reading frames (ORFs) with unknown function were detected, including two unique to the pol mitotype (orf122 and orf132). At least five rearrangement events are required to account for the structural differences between the pol and nap sequences. The CMS-related orf224 neighboring region (∼5 kb) rearranged twice. PCR profiling based on mitotype-specific primer pairs showed that both mitotypes are present in B. napus cultivars. Quantitative PCR showed that the pol cytoplasm consists mainly of the pol mitotype, and the nap mitotype is the main genome of nap cytoplasm. Large variation in the copy number ratio of mitotypes was found, even among cultivars sharing the same cytoplasm. The coexistence of mitochondrial mitotypes and substoichiometric shifting can explain the emergence of CMS in B. napus.
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