ArticlePLoS pathogens2011
The moving junction protein RON8 facilitates firm attachment and host cell invasion in Toxoplasma gondii.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- EtSERPIN1 binding with chicken ANXA2 is essential for Eimeria tenella attachment and invasion process.Veterinary research · 2025Article
- PLASMOpred: A Machine Learning-Based Web Application for Predicting Antimalarial Small Molecules Targeting the Apical Membrane Antigen 1-Rhoptry Neck Protein 2 Invasion Complex.Pharmaceuticals (Basel, Switzerland) · 2025Article
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- Effective factors in the pathogenesis ofHeliyon · 2024Review
- Toxoplasma sortilin interacts with secretory proteins and it is critical for parasite proliferation.Parasites & vectors · 2024Article
- A Coccidia-Specific Phosphate Transporter Is Essential for the Growth of Toxoplasma gondii Parasites.Microbiology spectrum · 2022Article
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- A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion.The Journal of infectious diseases · 2020Article
- TheBiology open · 2019Article
- How does Toxoplama gondii invade host cells?The Journal of veterinary medical science · 2018Review
- SURGE complex of Plasmodium falciparum in the rhoptry-neck (SURFIN4.2-RON4-GLURP) contributes to merozoite invasion.PloS one · 2018Article
- RON4Scientific reports · 2017Article
- Gliding motility powers invasion and egress in Apicomplexa.Nature reviews. Microbiology · 2017Review
- Article
- Multilevel Precision-Based Rational Design of Chemical Inhibitors Targeting the Hydrophobic Cleft of Toxoplasma gondii Apical Membrane Antigen 1 (AMA1).Genomics & informatics · 2016Article
- Identification and Characterization of the Rhoptry Neck Protein 2 in Babesia divergens and B. microti.Infection and immunity · 2016Article
- The Conoid Associated Motor MyoH Is Indispensable for Toxoplasma gondii Entry and Exit from Host Cells.PLoS pathogens · 2016Article
- Article
- Lytic Cycle of Toxoplasma gondii: 15 Years Later.Annual review of microbiology · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The apicomplexan moving junction (MJ) is a highly conserved structure formed during host cell entry that anchors the invading parasite to the host cell and serves as a molecular sieve of host membrane proteins that protects the parasitophorous vacuole from host lysosomal destruction. While recent work in Toxoplasma and Plasmodium has reinforced the composition of the MJ as an important association of rhoptry neck proteins (RONs) with micronemal AMA1, little is known of the precise role of RONs in the junction or how they are targeted to the neck subcompartment. We report the first functional analysis of a MJ/RON protein by disrupting RON8 in T. gondii. Parasites lacking RON8 are severely impaired in both attachment and invasion, indicating that RON8 enables the parasite to establish a firm clasp on the host cell and commit to invasion. The remaining junction components frequently drag in trails behind invading knockout parasites and illustrate a malformed complex without RON8. Complementation of Δron8 parasites restores invasion and reveals a processing event at the RON8 C-terminus. Replacement of an N-terminal region of RON8 with a mCherry reporter separates regions within RON8 that are necessary for rhoptry targeting and complex formation from those required for function during invasion. Finally, the invasion defects in Δron8 parasites seen in vitro translate to radically impaired virulence in infected mice, promoting a model in which RON8 has a crucial and unprecedented task in committing Toxoplasma to host cell entry.
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