ArticleFrontiers in psychiatry2010
Membrane-Bound Catechol-O-Methyl Transferase in Cortical Neurons and Glial Cells is Intracellularly Oriented.
Article in Frontiers in psychiatry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Characterization of catecholaminergic and serotonergic pathways in the trachea of the chicken and turkey.Poultry science · 2026Article
- Ontogeny of Catechol-O-Methyltransferase Expression in the Rat Prefrontal Cortex: Effects of Methamphetamine Exposure.Developmental neuroscience · 2026Article
- Integrated multiplexed assays of variant effect reveal determinants of catechol-O-methyltransferase gene expression.Molecular systems biology · 2024Article
- Characterization by Gene Expression Analysis of Two Groups of Dopaminergic Cells Isolated from the Mouse Olfactory Bulb.Biology · 2023Article
- Discovery of Small Molecules as Membrane-Bound Catechol-Pharmaceuticals (Basel, Switzerland) · 2021Article
- Motivational learning biases are differentially modulated by genetic determinants of striatal and prefrontal dopamine function.Journal of neural transmission (Vienna, Austria : 1996) · 2021Article
- Associations between catechol-O-methyltransferase (COMT) genotypes at rs4818 and rs4680 and gene expression in human dorsolateral prefrontal cortex.Experimental brain research · 2020Article
- Synaptic Effects of Dopamine Breakdown and Their Relation to Schizophrenia-Linked Working Memory Deficits.Frontiers in synaptic neuroscience · 2018Article
- Subsecond Regulation of Synaptically Released Dopamine by COMT in the Olfactory Bulb.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Neural correlates of reward processing in adults with 22q11 deletion syndrome.Journal of neurodevelopmental disorders · 2016Article
- Temporal profiles dissociate regional extracellular ethanol versus dopamine concentrations.ACS chemical neuroscience · 2015Article
- Genetic variation in COMT activity impacts learning and dopamine release capacity in the striatum.Learning & memory (Cold Spring Harbor, N.Y.) · 2014Article
- Gene-sex interactions in schizophrenia: focus on dopamine neurotransmission.Frontiers in behavioral neuroscience · 2014Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catechol-O-methyl transferase (COMT) is involved in the inactivation of dopamine in brain regions in which the dopamine transporter (DAT1) is sparsely expressed. The membrane-bound isoform of COMT (MB-COMT) is the predominantly expressed form in the mammalian central nervous system (CNS). It has been a matter of debate whether in neural cells of the CNS the enzymatic domain of MB-COMT is oriented toward the cytoplasmic or the extracellular compartment. Here we used live immunocytochemistry on cultured neocortical neurons and glial cells to investigate the expression and membrane orientation of native COMT and of transfected MB-COMT fused to green fluorescent protein (GFP). After live staining, COMT immunoreactivity was reliably detected in both neurons and glial cells after permeabilization, but not on unpermeabilized cells. Similarly, autofluorescence of COMT-GFP fusion protein and antibody fluorescence showed overlap only in permeabilized neurons. Our data provide converging evidence for an intracellular membrane orientation of MB-COMT in neurons and glial cells, suggesting the presence of a DAT1-independent postsynaptic uptake mechanism for dopamine, prior to its degradation via COMT.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.