ArticleFrontiers in physiology2010
A membrane protein/signaling protein interaction network for Arabidopsis version AMPv2.
Article in Frontiers in physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 146 citations in OpenAlex.
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- Integrated Membrane Yeast Two-Hybrid System for the Analysis of Membrane Protein Complexes.Bio-protocol · 2025Article
- Research progress and application strategies of sugar transport mechanisms in rice.Frontiers in plant science · 2024Review
- A cornichon protein controls polar localization of the PINA auxin transporter in Physcomitrium patens.Development (Cambridge, England) · 2023Article
- TheInternational journal of molecular sciences · 2023Article
- MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice.Plant biotechnology journal · 2023Article
- The C-terminus of the cargo receptor Erv14 affects COPII vesicle formation and cargo delivery.Journal of cell science · 2023Article
- Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress.Plant physiology · 2023Article
- Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.The Plant cell · 2022Article
- Ammonium transporter 1 increases rice resistance to sheath blight by promoting nitrogen assimilation and ethylene signalling.Plant biotechnology journal · 2022Article
- Feedback inhibition of AMT1 NHBMC biology · 2020Article
- The Regulatory Network ofInternational journal of molecular sciences · 2020Article
- The Arabidopsis Plastidial Glucose-6-Phosphate Transporter GPT1 is Dually Targeted to Peroxisomes via the Endoplasmic Reticulum.The Plant cell · 2020Article
- Broad-Spectrum Amino Acid Transporters ClAAP3 and ClAAP6 Expressed in Watermelon Fruits.International journal of molecular sciences · 2019Article
- Cucumber Mildew Resistance Locus O Interacts with Calmodulin and Regulates Plant Cell Death Associated with Plant Immunity.International journal of molecular sciences · 2019Article
- HSFA2 Functions in the Physiological Adaptation of Undifferentiated Plant Cells to Spaceflight.International journal of molecular sciences · 2019Article
- Inhibition of OsSWEET11 function in mesophyll cells improves resistance of rice to sheath blight disease.Molecular plant pathology · 2018Article
- Plant genome and transcriptome annotations: from misconceptions to simple solutions.Briefings in bioinformatics · 2018Article
- Hub Protein Controversy: Taking a Closer Look at Plant Stress Response Hubs.Frontiers in plant science · 2018Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
24 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interactions between membrane proteins and the soluble fraction are essential for signal transduction and for regulating nutrient transport. To gain insights into the membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list of 8,383 representing membrane and signaling proteins from Arabidopsis thaliana were cloned into a Gateway-compatible vector. The mating-based split ubiquitin system was used to screen for potential protein-protein interactions (pPPIs) among 490 Arabidopsis ORFs. A binary robotic screen between 142 receptor-like kinases (RLKs), 72 transporters, 57 soluble protein kinases and phosphatases, 40 glycosyltransferases, 95 proteins of various functions, and 89 proteins with unknown function detected 387 out of 90,370 possible PPIs. A secondary screen confirmed 343 (of 386) pPPIs between 179 proteins, yielding a scale-free network (r(2) = 0.863). Eighty of 142 transmembrane RLKs tested positive, identifying 3 homomers, 63 heteromers, and 80 pPPIs with other proteins. Thirty-one out of 142 RLK interactors (including RLKs) had previously been found to be phosphorylated; thus interactors may be substrates for respective RLKs. None of the pPPIs described here had been reported in the major interactome databases, including potential interactors of G-protein-coupled receptors, phospholipase C, and AMT ammonium transporters. Two RLKs found as putative interactors of AMT1;1 were independently confirmed using a split luciferase assay in Arabidopsis protoplasts. These RLKs may be involved in ammonium-dependent phosphorylation of the C-terminus and regulation of ammonium uptake activity. The robotic screening method established here will enable a systematic analysis of membrane protein interactions in fungi, plants and metazoa.
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