ArticleCell research2011
Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601.
Article in Cell research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 70 citations in OpenAlex.
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
- Core genome sequencing and genotyping of Leptospira interrogans in clinical samples by target capture sequencing.BMC infectious diseases · 2023Article
- Complete Genome Sequencing ofPathogens (Basel, Switzerland) · 2021Article
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- Comparative genomic analysis of Chinese human leptospirosis vaccine strain and circulating isolate.Human vaccines & immunotherapeutics · 2020Article
- Genus-wide Leptospira core genome multilocus sequence typing for strain taxonomy and global surveillance.PLoS neglected tropical diseases · 2019Article
- Genomic Variability among Field Isolates and Laboratory-Adapted Strains ofInternational journal of microbiology · 2018Article
- Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates.International journal of molecular sciences · 2017Review
- A Novel Pan-Genome Reverse Vaccinology Approach Employing a Negative-Selection Strategy for Screening Surface-Exposed Antigens againstFrontiers in microbiology · 2017Article
- Genomic Analysis of a New Serovar ofFrontiers in microbiology · 2017Article
- Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline.Molecular & cellular proteomics : MCP · 2016Article
- Genomic survey and expression analysis of DNA repair genes in the genus Leptospira.Molecular genetics and genomics : MGG · 2016Article
- Analysis of LexA binding sites and transcriptomics in response to genotoxic stress in Leptospira interrogans.Nucleic acids research · 2016Article
- Whole genome sequencing revealed host adaptation-focused genomic plasticity of pathogenic Leptospira.Scientific reports · 2016Article
- Whole Genome Shotgun Sequencing Shows Selection on Leptospira Regulatory Proteins During in vitro Culture Attenuation.The American journal of tropical medicine and hygiene · 2016Article
- Article
- Distribution of Plasmids in Distinct Leptospira Pathogenic Species.PLoS neglected tropical diseases · 2015Article
- Comparative genomic analyses of transport proteins encoded within the genomes of Leptospira species.Microbial pathogenesis · 2015Article
- Article
- Identification of three extra-chromosomal replicons in Leptospira pathogenic strain and development of new shuttle vectors.BMC genomics · 2015Article
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Authors and funding
17 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China. We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L. interrogans serovar Lai strain 56601. The complete genome sequence of the IPAV strain was determined and used to compare with, and then rectify and reannotate the genome sequence of strain 56601. Aside from their highly similar genomic structure and gene order, a total of 33 insertions, 53 deletions and 301 single-nucleotide variations (SNVs) were detected throughout the genome of IPAV directly affecting 101 genes, either in their 5' upstream region or within their coding region. Among them, the majority of the 44 functional genes are involved in signal transduction, stress response, transmembrane transport and nitrogen metabolism. Comparative proteomic analysis based on quantitative liquid chromatography (LC)-MS/MS data revealed that among 1 627 selected pairs of orthologs, 174 genes in the IPAV strain were upregulated, with enrichment mainly in classes of energy production and lipid metabolism. In contrast, 228 genes in strain 56601 were upregulated, with the majority enriched in the categories of protein translation and DNA replication/repair. The combination of genomic and proteomic approaches illustrated that altered expression or mutations in critical genes, such as those encoding a Ser/Thr kinase, carbon-starvation protein CstA, glutamine synthetase, GTP-binding protein BipA, ribonucleotide-diphosphate reductase and phosphate transporter, and alterations in the translational profile of lipoproteins or outer membrane proteins are likely to account for the virulence attenuation in strain IPAV.
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