ArticleNature chemical biology2011
Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
Article in Nature chemical biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
- Spotlight on the Nucleotide: Solid-State NMR for the Investigation of ATP Hydrolysis in the ATPase SmsC.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Mechanism of sensor kinase CitA transmembrane signaling.Nature communications · 2025Article
- Initial Primer Synthesis of a DNA Primase Monitored by Real-Time NMR Spectroscopy.Journal of the American Chemical Society · 2024Article
- The pathway for coenzyme M biosynthesis in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structure of membrane diacylglycerol kinase in lipid bilayers.Communications biology · 2021Article
- Real-time nuclear magnetic resonance spectroscopy in the study of biomolecular kinetics and dynamics.Magnetic resonance (Gottingen, Germany) · 2021Review
- Light-Induced Uncaging for Time-Resolved Observations of Biochemical Reactions by MAS NMR Spectroscopy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR.Scientific reports · 2019Article
- Structural Basis of TRPV4 N Terminus Interaction with Syndapin/PACSIN1-3 and PIPStructure (London, England : 1993) · 2018Article
- Article
- Single particle tracking-based reaction progress kinetic analysis reveals a series of molecular mechanisms of cetuximab-induced EGFR processes in a single living cell.Chemical science · 2017Article
- Active Site Hydrophobicity and the Convergent Evolution of Paraoxonase Activity in Structurally Divergent Enzymes: The Case of Serum Paraoxonase 1.Journal of the American Chemical Society · 2017Article
- Coupled ATPase-adenylate kinase activity in ABC transporters.Nature communications · 2016Article
- Diacylglycerol Kinases (DGKs): Novel Targets for Improving T Cell Activity in Cancer.Frontiers in cell and developmental biology · 2016Review
- BSH-CP based 3D solid-state NMR experiments for protein resonance assignment.Journal of biomolecular NMR · 2014Article
- Recent advances in solid-state nuclear magnetic resonance techniques to quantify biomolecular dynamics.Analytical chemistry · 2014Review
- A model for the interfacial kinetics of phospholipase D activity on long-chain lipids.Biophysical journal · 2013Article
- Article
- Hyperconjugation-mediated solvent effects in phosphoanhydride bonds.The journal of physical chemistry. A · 2012Article
- Prokaryotic diacylglycerol kinase and undecaprenol kinase.Annual review of biophysics · 2012Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The simultaneous observation of interdependent reactions within different phases as catalyzed by membrane-bound enzymes is still a challenging task. One such enzyme, the Escherichia coli integral membrane protein diacylglycerol kinase (DGK), is a key player in lipid regulation. It catalyzes the generation of phosphatidic acid within the membrane through the transfer of the γ-phosphate from soluble MgATP to membrane-bound diacylglycerol. We demonstrate that time-resolved (31)P magic angle spinning NMR offers a unique opportunity to simultaneously and directly detect both ATP hydrolysis and diacylglycerol phosphorylation. This experiment demonstrates that solid-state NMR provides a general approach for the kinetic analysis of coupled reactions at the membrane interface regardless of their compartmentalization. The enzymatic activity of DGK was probed with different lipid substrates as well as ATP analogs. Our data yield conclusions about intersubunit cooperativity, reaction stoichiometries and phosphoryl transfer mechanism and are discussed in the context of known structural data.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.