ArticleEpidemiology (Cambridge, Mass.)2010
Treatment of batch in the detection, calibration, and quantification of immunoassays in large-scale epidemiologic studies.
Article in Epidemiology (Cambridge, Mass.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Periconception Maternal-Serum Vitamin D, Vaginal Bleeding, and Subchorionic Hemorrhage in Early Pregnancy.Nutrients · 2026Trial
- Antimüllerian hormone and pregnancy loss from the Effects of Aspirin in Gestation and Reproduction trial.Fertility and sterility · 2016Trial
- Is Anti-Müllerian Hormone Associated With Fecundability? Findings From the EAGeR Trial.The Journal of clinical endocrinology and metabolism · 2015Trial
- Spousal bereavement enhances proinflammatory cytokine production to acute, psychological stress.Psychoneuroendocrinology · 2025Article
- Patterns of Sleep Duration and Metabolic Biomarkers Across the Menstrual Cycle.The Journal of clinical endocrinology and metabolism · 2025Article
- Systematic Review of Kidney Injury Biomarkers for the Evaluation of CKD of Uncertain Etiology.Kidney international reports · 2024Article
- Cytokine Patterns in Maternal Serum From First Trimester to Term and Beyond.Frontiers in immunology · 2021Article
- Recalled maternal lifestyle behaviors associated with anti-müllerian hormone of adult female offspring.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells.Immune network · 2020Article
- Combining Biomarker Calibration Data to Reduce Measurement Error.Epidemiology (Cambridge, Mass.) · 2019Article
- Body Mass Index and Type 2 Collagen Turnover in Individuals After Anterior Cruciate Ligament Reconstruction.Journal of athletic training · 2019Article
- Identification of Sex-Specific Transcriptome Responses to Polychlorinated Biphenyls (PCBs).Scientific reports · 2019Article
- A computational solution to improve biomarker reproducibility during long-term projects.PloS one · 2019Article
- Temporal reliability of serum soluble and endogenous secretory receptors for advanced glycation end-products (sRAGE and esRAGE) in healthy women.Cancer causes & control : CCC · 2018Article
- MINT: a multivariate integrative method to identify reproducible molecular signatures across independent experiments and platforms.BMC bioinformatics · 2017Article
- Serum caffeine and paraxanthine concentrations and menstrual cycle function: correlations with beverage intakes and associations with race, reproductive hormones, and anovulation in the BioCycle Study.The American journal of clinical nutrition · 2016Article
- A Method and On-Line Tool for Maximum Likelihood Calibration of Immunoblots and Other Measurements That Are Quantified in Batches.PloS one · 2016Article
- Dietary Carbohydrate Intake Does Not Impact Insulin Resistance or Androgens in Healthy, Eumenorrheic Women.The Journal of clinical endocrinology and metabolism · 2015Article
- The relationship between blood IL-12p40 level and melanoma progression.International journal of cancer · 2015Article
- Biological variability in serum anti-Müllerian hormone throughout the menstrual cycle in ovulatory and sporadic anovulatory cycles in eumenorrheic women.Human reproduction (Oxford, England) · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
unlabelledMany laboratory assays measure biomarkers via a 2-stage process. Direct measurement yields relative measures that are subsequently transformed to the unit of interest by using a calibration experiment. The calibration experiment is performed within the main experiment and uses a validation set for which true values are known and relative values are measured by assays to estimate the relation between relative and absolute values. Immunoassays, polymerase chain reaction, and chromatographic approaches are among assays performed in this manner.
methodsFor studies with multiple batches, data from more than a single calibration experiment are available. Conventionally, calibration of assays based on the standard curve is performed specific to each batch; the calibration experiment from each batch is used to calibrate each batch independently. This batch-specific approach incorporates batch variability but, due to the small number of calibration measurements in each batch, may not be best suited for this purpose.
resultsMixed-effects models have been described to address interassay variability and to provide a measure of quality assurance. Conversely, when interbatch variability is negligible, a model that does not incorporate batch effect may be used to estimate an overall calibration curve.
conclusionWe explore approaches for use of calibration data in studies with many batches. Using a real data example with biomarker and outcome information, we show that risk estimates may vary depending on the calibration approach used. We demonstrate the potential for bias when using simulations. Under minimal interbatch variability, as seen in our data, conventional batch-specific calibration does not best use information available in the data and results in attenuated risk estimates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.