ArticleProceedings of the National Academy of Sciences of the United States of America2011
Single-cell gene-expression profiling reveals qualitatively distinct CD8 T cells elicited by different gene-based vaccines.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 92 citations in OpenAlex.
- A human vaccine strategy based on chimpanzee adenoviral and MVA vectors that primes, boosts, and sustains functional HCV-specific T cell memory.Science translational medicine · 2014Trial
- Persistence of EBV antigen-specific CD8 T cell clonotypes during homeostatic immune reconstitution in cancer patients.PloS one · 2013Trial
- Single-cell expression quantitative trait loci (eQTL) analysis of SLE-risk loci in lupus patient monocytes.Arthritis research & therapy · 2021Article
- Single-Cell Analysis of Antigen-Specific CD8+ T-Cell Transcripts Reveals Profiles Specific to mRNA or Adjuvanted Protein Vaccines.Frontiers in immunology · 2021Article
- Heterologous Prime Boost Vaccination Induces Protective Melanoma-Specific CD8Molecular therapy oncolytics · 2020Article
- Killer-like receptors and GPR56 progressive expression defines cytokine production of human CD4Nature communications · 2019Article
- A deadly dance: the choreography of host-pathogen interactions, as revealed by single-cell technologies.Nature communications · 2018Review
- Single-cell gene expression patterns in lupus monocytes independently indicate disease activity, interferon and therapy.Lupus science & medicine · 2017Article
- A rare subset of skin-tropic regulatory T cells expressing Il10/Gzmb inhibits the cutaneous immune response.Scientific reports · 2016Article
- Exploiting Single-Cell Quantitative Data to Map Genetic Variants Having Probabilistic Effects.PLoS genetics · 2016Article
- Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.The Journal of experimental medicine · 2016Article
- Article
- Application of an RNA amplification method for reliable single-cell transcriptome analysis.BioTechniques · 2015Article
- Transcriptional profiles reveal a stepwise developmental program of memory CD8(+) T cell differentiation.Vaccine · 2015Article
- Article
- Heterogeneity in immune responses: from populations to single cells.Trends in immunology · 2014Review
- Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells.Nature biotechnology · 2014Review
- Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue.Nature methods · 2014Article
- Mixture models for single-cell assays with applications to vaccine studies.Biostatistics (Oxford, England) · 2014Article
- Systems-level analysis of innate immunity.Annual review of immunology · 2014Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD8 T cells play a key role in mediating protective immunity against selected pathogens after vaccination. Understanding the mechanism of this protection is dependent upon definition of the heterogeneity and complexity of cellular immune responses generated by different vaccines. Here, we identify previously unrecognized subsets of CD8 T cells based upon analysis of gene-expression patterns within single cells and show that they are differentially induced by different vaccines. Three prime-boost vector combinations encoding HIV Env stimulated antigen-specific CD8 T-cell populations of similar magnitude, phenotype, and functionality. Remarkably, however, analysis of single-cell gene-expression profiles enabled discrimination of a majority of central memory (CM) and effector memory (EM) CD8 T cells elicited by the three vaccines. Subsets of T cells could be defined based on their expression of Eomes, Cxcr3, and Ccr7, or Klrk1, Klrg1, and Ccr5 in CM and EM cells, respectively. Of CM cells elicited by DNA prime-recombinant adenoviral (rAd) boost vectors, 67% were Eomes(-) Ccr7(+) Cxcr3(-), in contrast to only 7% and 2% stimulated by rAd5-rAd5 or rAd-LCMV, respectively. Of EM cells elicited by DNA-rAd, 74% were Klrk1(-) Klrg1(-)Ccr5(-) compared with only 26% and 20% for rAd5-rAd5 or rAd5-LCMV. Definition by single-cell gene profiling of specific CM and EM CD8 T-cell subsets that are differentially induced by different gene-based vaccines will facilitate the design and evaluation of vaccines, as well as enable our understanding of mechanisms of protective immunity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.