ArticleInfection and immunity2011
Identification of in vivo-induced antigens including an RTX family exoprotein required for uropathogenic Escherichia coli virulence.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Comprehensive analysis of Escherichia coli in bovine urinary tract infections: serotypes, antibiotic resistance, and virulence gene profiles.Scientific reports · 2025Article
- Review
- Urinary Tract Infections Caused by UropathogenicInternational journal of molecular sciences · 2023Review
- The PapB/FocB family protein TosR acts as a positive regulator of flagellar expression and is required for optimal virulence of uropathogenicFrontiers in microbiology · 2023Article
- Cranberry Polyphenols and Prevention against Urinary Tract Infections: Relevant Considerations.Molecules (Basel, Switzerland) · 2020Review
- Survey and Sequence Characterization of Bovine Mastitis-AssociatedFrontiers in veterinary science · 2020Article
- Pathogenicity Factors of Genomic Islands in Intestinal and ExtraintestinalFrontiers in microbiology · 2020Review
- Effective Pro-Inflammatory Induced Activity of GALT, a Conserved Antigen inFrontiers in cellular and infection microbiology · 2018Article
- Article
- Siderophore vaccine conjugates protect against uropathogenic Escherichia coli urinary tract infection.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Drug and Vaccine Development for the Treatment and Prevention of Urinary Tract Infections.Microbiology spectrum · 2016Review
- Regulation of Expression of Uropathogenic Escherichia coli Nonfimbrial Adhesin TosA by PapB Homolog TosR in Conjunction with H-NS and Lrp.Infection and immunity · 2016Article
- Multidrug- and Extensively Drug-Resistant UropathogenicFrontiers in microbiology · 2016Article
- Structure, Function, and Assembly of Adhesive Organelles by Uropathogenic Bacteria.Microbiology spectrum · 2015Review
- Virulence and Fitness Determinants of Uropathogenic Escherichia coli.Microbiology spectrum · 2015Review
- Article
- A conserved PapB family member, TosR, regulates expression of the uropathogenic Escherichia coli RTX nonfimbrial adhesin TosA while conserved LuxR family members TosE and TosF suppress motility.Infection and immunity · 2014Article
- Assessing the exoproteome of marine bacteria, lesson from a RTX-toxin abundantly secreted by Phaeobacter strain DSM 17395.PloS one · 2014Article
- Identification of Salmonella enterica serovar Pullorum antigenic determinants expressed in vivo.Infection and immunity · 2013Article
- Immunization with the yersiniabactin receptor, FyuA, protects against pyelonephritis in a murine model of urinary tract infection.Infection and immunity · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Uncomplicated urinary tract infections (UTI) are caused most commonly by uropathogenic Escherichia coli (UPEC). Whole-genome screening approaches, including transcriptomic, proteomic, and signature-tagged mutagenesis, have shown that UPEC highly expresses or requires genes for translational machinery, capsule, lipopolysaccharide, type 1 fimbriae, and iron acquisition systems during UTI. To identify additional genes expressed by UPEC during UTI, an immunoscreening approach termed in vivo-induced antigen technology (IVIAT) was employed to identify antigens produced during experimental infection that are not produced during in vitro culture. An inducible protein expression library, constructed from genomic DNA isolated from UPEC strain CFT073, was screened using exhaustively adsorbed pooled sera from 20 chronically infected female CBA/J mice. Using this approach, we identified 93 antigens induced by UPEC in vivo. A representative subset of these genes was tested by quantitative PCR for expression by CFT073 in vivo and during growth in human urine or LB medium in vitro; proWX, narJI, lolA, lolD, tosA (upxA), c2432, katG, ydhX, kpsS, and yddQ were poorly expressed in vitro but highly expressed in vivo. Of these, tosA, a gene encoding a predicted repeat-in-toxin family member, was expressed exclusively during UTI. Deletion of tosA in UPEC strain CFT073 resulted in significant attenuation in bladder and kidney infections during ascending UTI. By screening for in vivo-induced antigens, we identified a novel UPEC virulence factor and additional proteins that could be useful as potential vaccine targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.