ArticleInfection and immunity2011
A Chlamydia trachomatis OmcB C-terminal fragment is released into the host cell cytoplasm and is immunogenic in humans.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Preclinical screen for protection efficacy of chlamydial antigens that are immunogenic in humans.Infection and immunity · 2023Article
- TargeTron Inactivation of Chlamydia trachomatisInfection and immunity · 2023Article
- Virulence Protein Pgp3 Is Insufficient To Mediate Plasmid-Dependent Infectivity of Chlamydia trachomatis.Infection and immunity · 2023Article
- The Small Molecule H89 InhibitsInfection and immunity · 2021Article
- Eukaryotic Cell Permeabilisation to Identify New Putative Chlamydial Type III Secretion System Effectors Secreted within Host Cell Cytoplasm.Microorganisms · 2020Article
- Chlamydial Plasmid-Dependent Pathogenicity.Trends in microbiology · 2017Review
- Analysis of CPAF mutants: new functions, new questions (the ins and outs of a chlamydial protease).Pathogens and disease · 2014Article
- Reassessing the role of the secreted protease CPAF in Chlamydia trachomatis infection through genetic approaches.Pathogens and disease · 2014Article
- Undressing of Waddlia chondrophila to enrich its outer membrane proteins to develop a new species-specific ELISA.New microbes and new infections · 2014Article
- A path forward for the chlamydial virulence factor CPAF.Microbes and infection · 2013Review
- Chlamydia trachomatis outer membrane complex protein B (OmcB) is processed by the protease CPAF.Journal of bacteriology · 2013Article
- Article
- Chlamydia trachomatis antigens recognized in women with tubal factor infertility, normal fertility, and acute infection.Obstetrics and gynecology · 2012Article
- Potential mechanisms for increased HIV-1 transmission across the endocervical epithelium during C. trachomatis infection.Current HIV research · 2012Review
- Article
- Localization of Chlamydia trachomatis hypothetical protein CT311 in host cell cytoplasm.Microbial pathogenesis · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Chlamydia trachomatis outer membrane complex protein B (OmcB) is an antigen with diagnostic and vaccine relevance. To further characterize OmcB, we generated antibodies against OmcB C-terminal (OmcBc) and N-terminal (OmcBn) fragments. Surprisingly, the anti-OmcBc antibody detected dominant signals in the host cell cytosol, while the anti-OmcBn antibody exclusively labeled intrainclusion signals in C. trachomatis-infected cells permeabilized with saponin. Western blot analyses revealed that OmcB was partially processed into OmcBc and OmcBn fragments. The processed OmcBc was released into host cell cytosol, while the OmcBn and remaining full-length OmcB were retained within the chlamydial inclusions. The organism-associated OmcB epitopes became detectable only after the C. trachomatis-infected cells were permeabilized with strong detergents such as SDS. However, the harsh permeabilization conditions also led to the leakage of the already secreted OmcBc and chlamydia-secreted protease (CPAF) out of the host cells. The OmcBc processing and release occurred in all biovars of C. trachomatis. Moreover, the released OmcBc but not the retained OmcBn was highly immunogenic in C. trachomatis-infected women, which is consistent with the concept that exposure of chlamydial proteins to host cell cytosol is accompanied by increased immunogenicity. These observations have provided important information for further exploring/optimizing OmcB as a target for the development of diagnosis methods and vaccines.
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