ArticleInfection and immunity2011
Genotype and phenotypes of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
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- Gut microbe Lactiplantibacillus plantarum undergoes different evolutionary trajectories between insects and mammals.BMC biology · 2022Article
- The microbial ecology of Escherichia coli in the vertebrate gut.FEMS microbiology reviews · 2022Review
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- Interplay between Bacterial Clones and Plasmids in the Spread of Antibiotic Resistance Genes in the Gut: Lessons from a Temporal Study in Veal Calves.Applied and environmental microbiology · 2021Article
- Temporal dynamics of the fecal microbiota in veal calves in a 6-month field trial.Animal microbiome · 2020Article
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- Integrated Experimental and Computational Analyses Reveal Differential Metabolic Functionality in Antibiotic-Resistant Pseudomonas aeruginosa.Cell systems · 2019Article
- A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters.Scientific reports · 2018Article
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- Evidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanisms.FEMS microbiology ecology · 2016Article
- Pathoadaptive Mutations of Escherichia coli K1 in Experimental Neonatal Systemic Infection.PloS one · 2016Article
- Post-Genomic Analysis of Members of the Family Vibrionaceae.Microbiology spectrum · 2015Review
- Mutations That Stimulate flhDC Expression in Escherichia coli K-12.Journal of bacteriology · 2015Article
- Commensal and Pathogenic Escherichia coli Metabolism in the Gut.Microbiology spectrum · 2015Article
- Factors that affect transfer of the IncI1 β-lactam resistance plasmid pESBL-283 between E. coli strains.PloS one · 2015Article
- Escherichia coli pathotypes occupy distinct niches in the mouse intestine.Infection and immunity · 2014Article
- The first steps of adaptation of Escherichia coli to the gut are dominated by soft sweeps.PLoS genetics · 2014Article
- An Escherichia coli Nissle 1917 missense mutant colonizes the streptomycin-treated mouse intestine better than the wild type but is not a better probiotic.Infection and immunity · 2014Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
We previously isolated a spontaneous mutant of Escherichia coli K-12, strain MG1655, following passage through the streptomycin-treated mouse intestine, that has colonization traits superior to the wild-type parent strain (M. P. Leatham et al., Infect. Immun. 73:8039-8049, 2005). This intestine-adapted strain (E. coli MG1655*) grew faster on several different carbon sources than the wild type and was nonmotile due to deletion of the flhD gene. We now report the results of several high-throughput genomic analysis approaches to further characterize E. coli MG1655*. Whole-genome pyrosequencing did not reveal any changes on its genome, aside from the deletion at the flhDC locus, that could explain the colonization advantage of E. coli MG1655*. Microarray analysis revealed modest yet significant induction of catabolic gene systems across the genome in both E. coli MG1655* and an isogenic flhD mutant constructed in the laboratory. Catabolome analysis with Biolog GN2 microplates revealed an enhanced ability of both E. coli MG1655* and the isogenic flhD mutant to oxidize a variety of carbon sources. The results show that intestine-adapted E. coli MG1655* is more fit than the wild type for intestinal colonization, because loss of FlhD results in elevated expression of genes involved in carbon and energy metabolism, resulting in more efficient carbon source utilization and a higher intestinal population. Hence, mutations that enhance metabolic efficiency confer a colonization advantage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.