ArticleInfection and immunity2011
Deletion of a cation transporter promotes lysis in Streptococcus pneumoniae.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Fluoride triggers lysis inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- P-type ATPase magnesium transporter MgtA acts as a dimer.Nature structural & molecular biology · 2025Article
- Draft genome sequence of '3 Biotech · 2024Article
- The bacterial tyrosine kinase system CpsBCD governs the length of capsule polymers.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transition of Serotype 35B Pneumococci From Commensal to Prevalent Virulent Strain in Children.Frontiers in cellular and infection microbiology · 2021Article
- TheApplied and environmental microbiology · 2020Article
- A Mn-sensing riboswitch activates expression of a Mn2+/Ca2+ ATPase transporter in Streptococcus.Nucleic acids research · 2019Article
- Analyzing Thermal Stability of Cell Membrane of Salmonella Using Time-Multiplexed Impedance Sensing.Biophysical journal · 2018Article
- Magnesium therapy improves outcome in Streptococcus pneumoniae meningitis by altering pneumolysin pore formation.British journal of pharmacology · 2017Article
- The magnesium transporter A is activated by cardiolipin and is highly sensitive to free magnesium in vitro.eLife · 2016Article
- Physiology, Genomics, and Pathway Engineering of an Ethanol-Tolerant Strain of Clostridium phytofermentans.Applied and environmental microbiology · 2015Article
- Spermidine biosynthesis and transport modulate pneumococcal autolysis.Journal of bacteriology · 2014Article
- Mycobacterium tuberculosis P-type ATPases: possible targets for drug or vaccine development.BioMed research international · 2014Review
- A live-attenuated pneumococcal vaccine elicits CD4+ T-cell dependent class switching and provides serotype independent protection against acute otitis media.EMBO molecular medicine · 2014Article
- Pyruvate oxidase influences the sugar utilization pattern and capsule production in Streptococcus pneumoniae.PloS one · 2013Article
- A novel initiation mechanism of death in Streptococcus pneumoniae induced by the human milk protein-lipid complex HAMLET and activated during physiological death.The Journal of biological chemistry · 2012Article
- Pneumococcal gene complex involved in resistance to extracellular oxidative stress.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae is a significant human pathogen which causes respiratory and serious invasive diseases. Mg(2+) is essential for life, and its concentration varies throughout the human body. Magnesium uptake plays an important role in the virulence of many bacterial pathogens. To study the Mg(2+) uptake of S. pneumoniae strain D39, a mutant was generated in SPD1383, a P-type ATPase with homology to the Salmonella Mg(2+) transporter MgtA, which has also been shown to be a Ca(2+) exporter in strain TIGR4. Under low-Ca(2+) conditions, mutation led to a growth defect in complex medium and the gene was nearly essential for growth under low-Mg(2+) conditions. Addition of Mg(2+) restored the normal growth of the mutant in all cases, but the addition of other divalent cations had no effect. Addition of Ca(2+), Mn(2+), and Zn(2+) in the presence of high Mg(2+) concentrations inhibited restoration of growth. The mutant was unable to proliferate in blood, which was also alleviated by the addition of Mg(2+). The protein was located in the membrane and produced in various S. pneumoniae strains and pathogenic streptococcal species. Surprisingly, mutation of the gene led to an elevated toxicity for endothelial cells. This was caused by an increased amount of pneumolysin in the medium, mediated by elevated lysis of the mutant. Thus, in this study, we uncovered a role for SPD1383 in Mg(2+) uptake and hypothesize that the protein is a Mg(2+/)Ca(2+) antiporter. Furthermore, a disturbance in Mg(2+) homeostasis seems to promote lysis of S. pneumoniae.
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