Evidence map›Paper›PMID 21421883›Full record

ArticleInvestigative ophthalmology & visual science2011

Rewiring integrin-mediated signaling and cellular response with the peripheral myelin protein 22 and epithelial membrane protein 2 components of the tetraspan web.

Shawn A Morales, David Telander, Lucia Notterpek, Madhuri Wadehra, Jonathan Braun, Lynn K Gordon

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. A novel function of p53: a gatekeeper of retinal detachment.The American journal of pathology · 2012
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Shawn A MoralesDepartment of Ophthalmology, University of California at Los Angeles, Los Angeles, California, USA.
David Telander
Lucia Notterpek
Madhuri Wadehra
Jonathan Braun
Lynn K Gordon
University of California, Los Angeles · USUniversity of California, Davis · USUniversity of Florida · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Gregory Darin Field, DAVID S WILLIAMS · 1985 to 2026
$16.8M
UCLA CLINICAL AND TRANSLATIONAL SCIENCE INSTITUTEUL1RR033176 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2011 to 2011
$15.5M
Novel Therapies to Prevent Blindness Caused by Proliferative VitreoretinopathyR01EY019909 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GORDON, LYNN K · 2010 to 2013
$1.5M
Abnormal EMPS Expression Affects Pregnancy OutcomeR03HD048540 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BRAUN, JONATHAN · 2005 to 2006
$305k
NCATS NIH HHS UL1 TR000124NCRR NIH HHS UL1 RR033176NEI NIH HHS P30 EY000331NEI NIH HHS P30 EY000331-45NEI NIH HHS R01 EY019909NEI NIH HHS R01 EY019909-03NICHD NIH HHS HD48540NICHD NIH HHS R03 HD048540
6 · The paper itself

Abstract

purposeIntegrin-mediated collagen gel contraction by ARPE-19 is an in vitro model for proliferative vitreoretinopathy (PVR), an aberrant wound healing response after retinal detachment or ocular trauma. Expression of the tetraspan protein epithelial membrane protein 2 (EMP2) controls gel contraction through FAK activation. Peripheral myelin protein 22 (PMP22), another member of the tetraspan web, is closely related to EMP2. The purpose of this study was to determine whether PMP22 also controls the contractile phase associated with PVR.

methodsIntegrin expression, adhesion, and protein expression were assessed, respectively, through flow cytometry, binding to collagen types I and IV, and Western blot analysis. Collagen gel contraction was assessed using an in vitro assay.

resultsOverexpression of PMP22 in ARPE-19 cells (ARPE-19/PMP22) resulted in increased collagen adhesion. Gel contraction, however, was reduced by greater than 50% in ARPE-19/PMP22 cells (P < 0.001). In contrast to the FAK activation observed by increasing EMP2 expression, PMP22 overexpression led to increased AKT activation. The decrease in gel contraction by the ARPE-19/PMP22 cells was partially reversed through either PMP22 siRNA or by blockade of AKT.

conclusionsRelative expression of EMP2 or PMP22 within the tetraspan web drives a cellular response toward a FAK- or AKT-dependent pathway, respectively. EMP2 and PMP22 differentially regulate collagen gel contraction in the ARPE-19 cell line. The implication of this finding adds a new dimension to the concept of the tetraspan web, in which the abundance of individual tetraspan family members differentially regulates signal transduction and the downstream cellular response.

Indexed as

Antibodies, MonoclonalBlotting, WesternCell AdhesionCell LineCollagen Type ICollagen Type IVFlow CytometryFocal Adhesion Kinase 1HumansIntegrinsMembrane GlycoproteinsModels, BiologicalMyelin ProteinsProto-Oncogene Proteins c-aktRetinal Pigment EpitheliumSignal TransductionAntibodies, MonoclonalCollagen Type ICollagen Type IVEMP2 protein, humanFocal Adhesion Kinase 1IntegrinsMembrane GlycoproteinsMyelin ProteinsPMP22 protein, humanProto-Oncogene Proteins c-aktPTK2 protein, human

Identifiers

PMID21421883
PMCPMC3262552
OpenAlexW2023902766

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.