ArticleInvestigative ophthalmology & visual science2011
ABCG2-dependent dye exclusion activity and clonal potential in epithelial cells continuously growing for 1 month from limbal explants.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 26 citations in OpenAlex.
- Limbal epithelial stem cell sheets from young donors have better regenerative potential.Scientific reports · 2022Article
- Comparative study of substrate free and amniotic membrane scaffolds for cultivation of limbal epithelial sheet.Scientific reports · 2018Article
- Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture.Cytotechnology · 2018Article
- Divergent effects of Wnt/β-catenin signaling modifiers on the preservation of human limbal epithelial progenitors according to culture condition.Scientific reports · 2017Article
- Limbal Stem Cell Deficiency and Treatment with Stem Cell Transplantation.Turkish journal of ophthalmology · 2017Review
- Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry.PloS one · 2017Article
- Differentiation of human embryonic stem cells into corneal epithelial progenitor cells under defined conditions.PloS one · 2017Article
- Tcf7l2 localization of putative stem/progenitor cells in mouse conjunctiva.American journal of physiology. Cell physiology · 2016Article
- Inhibition of TGFβ cell signaling for limbal explant culture in serumless, defined xeno-free conditions.Experimental eye research · 2016Article
- Corneal recovery in a rabbit limbal stem cell deficiency model by autologous grafts of tertiary outgrowths from cultivated limbal biopsy explants.Molecular vision · 2016Article
- Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures.Stem cell reviews and reports · 2014Article
- Transcriptional dissection of human limbal niche compartments by massive parallel sequencing.PloS one · 2013Article
- Atomic force microscopy analysis of progenitor corneal epithelial cells fractionated by a rapid centrifugation isolation technique.PloS one · 2013Article
- CD133 is a marker for long-term repopulating murine epidermal stem cells.The Journal of investigative dermatology · 2012Article
- 25 years of epidermal stem cell research.The Journal of investigative dermatology · 2012Review
- [Perspectives and current state in limbal stem cell transplantation].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2011Review
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4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo determine changes in ABCG2-transport-dependent dye exclusion in outgrowths from limbal explants.
methodsHuman or rabbit limbal strips were deposited onto inserts. Over a month, the segments were twice transferred to new inserts. Fresh tissue (FT) cells, obtained by sequential dispase-trypsin digestion and the cells growing from the explant cultures, were characterized for ABCG2-dependent efflux by flow cytometry using a newly identified substratum, JC1. Rabbit cells were sorted into JC1-excluding (JC1(low)) and main (JC1(main)) cohorts and seeded with feeder 3T3 cells to determine colony formation efficiency (CFE).
resultsThe JC1(low) cells were all Hoechst 33342-excluding cells and vice versa, establishing the physical equivalence between JC1(low) and the side population (SP). JC1(low) cell content was reduced by three ABCG2-specific inhibitors: FTC, Ko143, and glafenine. JC1(low) percentiles for the fresh human and rabbit cells were 1.4% and 4.1% and CFEs for rabbit JC1(low) and JC1(main) were 1.2% and 5.3%. In contrast, the respective JC1(low) percentiles in the first and second outgrowths were 19.5% and 27.4% and 25.8% and 32.5%, and the rabbit JC1(low) and JC1(main) CFEs were 12.3% and 0.9%. Thus, although in FT the contribution of the JC1(low) cohort to the CFE is minimal, in the explant culture the phenotype incorporates >80% of the CFE.
conclusionsABCG2-dependent dye exclusion undergoes a large expansion in explant culture and becomes associated with a high CFE. The transport increase is more pronounced at late outgrowth times, suggesting permanence of stem cells within the explant.
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