ArticleInvestigative ophthalmology & visual science2011
Entrapment of conjunctival goblet cells by desiccation-induced cornification.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
- Effects of Dry Eye Therapies on Environmentally Induced Ocular Surface Disease.American journal of ophthalmology · 2015Trial
- Review
- Conjunctivitis in Adults with Atopic Dermatitis Treated with Dupilumab: An Observational Study of Clinical Characteristics, Symptomatology, and Treatment.Advances in therapy · 2025Observational
- IL-17 Producing Lymphocytes Cause Dry Eye and Corneal Disease With Aging in RXRα Mutant Mouse.Frontiers in medicine · 2022Article
- Corneal epithelium in keratoconus underexpresses active NRF2 and a subset of oxidative stress-related genes.PloS one · 2022Article
- Inflammation and dry eye disease-where are we?International journal of ophthalmology · 2022Review
- Animal models of dry eye disease: Useful, varied and evolving (Review).Experimental and therapeutic medicine · 2021Review
- Aged Mice Devoid of the MInternational journal of molecular sciences · 2021Article
- A novel transillumination meibography device for in vivo imaging of mouse meibomian glands.The ocular surface · 2021Article
- Defining Dry Eye from a Clinical Perspective.International journal of molecular sciences · 2020Review
- The cornea in keratoconjunctivitis sicca.Experimental eye research · 2020Review
- The Lacritin-Syndecan-1-Heparanase Axis in Dry Eye Disease.Advances in experimental medicine and biology · 2020Review
- Suppression of Th1-Mediated Keratoconjunctivitis Sicca by Lifitegrast.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2018Article
- Role of mucins in lung homeostasis: regulated expression and biosynthesis in health and disease.Biochemical Society transactions · 2018Review
- [Blepharitis component of dry eye syndrome].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2018Review
- The Pathophysiology of Dry Eye Disease: What We Know and Future Directions for Research.Ophthalmology · 2017Review
- Effects of Aging in Dry Eye.International ophthalmology clinics · 2017Review
- What We Have Learned From Animal Models of Dry Eye.International ophthalmology clinics · 2017Article
- Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress.PloS one · 2017Article
- Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo evaluate the effects of desiccating stress on conjunctival goblet cell density and morphology and the expression of cornified envelope precursors by the ocular surface epithelia.
methodsExperimental dry eye (EDE) was created in C57BL/6 mice. Real-time PCR evaluated the expression of cornified envelope (CE) precursor proteins (involucrin and small proline-rich [Sprr] -1a, -1b, -2a, -2b, -2f, and -2g proteins), the cross-linking transglutaminase 1 enzyme (Tg-1) and Muc5AC mRNA transcripts by the ocular surface epithelia. Laser scanning confocal microscopy evaluated the expression of the CE precursor proteins Tg-1 and Muc5AC in cryosections. Tg-1 activity was measured by a fluorescein cadaverine assay. Muc5AC concentration was measured by ELISA.
resultsLevels of involucrin; Sprr-1a, -1b, -2a, -2b, -2f, and -2g; and Tg1-1 mRNA transcripts in ocular surface tissues increased in response to desiccating stress. Expression and activity of Tg in the conjunctiva markedly increased after EDE. Desiccating stress caused progressive loss of mucin-filled goblet cells. The apical portion of the remaining conjunctival goblet cells became entrapped by adjacent stratified apical epithelia expressing increased levels of cornified envelope precursors.
conclusionsExposure to desiccating stress stimulates ocular surface epithelia to produce cornified envelope precursors and the tissue transglutaminase enzyme that cross-links them. This effect is accompanied by loss of mucin-filled goblet cells and entrapment of mucin contents in the remaining ones by cornifying cells that block the egress of mucin contents to the ocular surface. This mechanism may contribute to the conjunctival mucin deficiency that develops in dry eye.
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