ArticleJournal of bacteriology2011
Role of maltose enzymes in glycogen synthesis by Escherichia coli.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803.FEBS letters · 2026Article
- Advances in bacterial synthesis pathways of bioflocculants and their applications in mine wastewater treatment.World journal of microbiology & biotechnology · 2026Review
- Demystifying polyphosphate-accumulating organisms relevant to wastewater treatment: A review of their phylogeny, metabolism, and detection.Environmental science and ecotechnology · 2024Review
- Role of MalQ Enzyme in a Reconstructed Maltose/Maltodextrin Pathway inMicroorganisms · 2024Article
- Enhanced production of maltobionic acid by a metabolically engineered Escherichia coli incapable of maltose utilization.Bioprocess and biosystems engineering · 2023Article
- Functional characterization of maltodextrin glucosidase for maltodextrin and glycogen metabolism in Vibrio vulnificus MO6-24/O.Archives of microbiology · 2022Article
- Characterization of a cold-adapted debranching enzyme and its role in glycogen metabolism and virulence of Vibrio vulnificus MO6-24/O.Journal of microbiology (Seoul, Korea) · 2022Article
- Production of Large-Ring Cyclodextrins by Amylomaltases.Molecules (Basel, Switzerland) · 2022Review
- Complete genome sequence of Arthrobacter sp. PAMC25564 and its comparative genome analysis for elucidating the role of CAZymes in cold adaptation.BMC genomics · 2021Article
- Enzymes of an alternative pathway of glucose metabolism in obligate methanotrophs.Scientific reports · 2021Article
- Comparison of Catalyzing Properties of Bacterial 4-α-Glucanotransferases Focusing on Their Cyclizing Activity.Journal of microbiology and biotechnology · 2021Article
- Starch and Glycogen Analyses: Methods and Techniques.Biomolecules · 2020Review
- Recent progress in the structure of glycogen serving as a durable energy reserve in bacteria.World journal of microbiology & biotechnology · 2020Review
- Glycogen Metabolism Impairment via Single Gene Mutation in theFrontiers in microbiology · 2020Article
- Article
- Omnipresent Maxwell's demons orchestrate information management in living cells.Microbial biotechnology · 2019Article
- Essentiality of the Maltase AmlE in Maltose Utilization and Its Transcriptional Regulation by the Repressor AmlR in the Acarbose-Producing BacteriumFrontiers in microbiology · 2019Article
- Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.Scientific reports · 2018Article
- Structure and Evolution of Glycogen Branching Enzyme N-Termini From Bacteria.Frontiers in microbiology · 2018Article
- Enzymes Required for Maltodextrin Catabolism in Enterococcus faecalis Exhibit Novel Activities.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutants with deletion mutations in the glg and mal gene clusters of Escherichia coli MC4100 were used to gain insight into glycogen and maltodextrin metabolism. Glycogen content, molecular mass, and branch chain distribution were analyzed in the wild type and in ΔmalP (encoding maltodextrin phosphorylase), ΔmalQ (encoding amylomaltase), ΔglgA (encoding glycogen synthase), and ΔglgA ΔmalP derivatives. The wild type showed increasing amounts of glycogen when grown on glucose, maltose, or maltodextrin. When strains were grown on maltose, the glycogen content was 20 times higher in the ΔmalP strain (0.97 mg/mg protein) than in the wild type (0.05 mg/mg protein). When strains were grown on glucose, the ΔmalP strain and the wild type had similar glycogen contents (0.04 mg/mg and 0.03 mg/mg protein, respectively). The ΔmalQ mutant did not grow on maltose but showed wild-type amounts of glycogen when grown on glucose, demonstrating the exclusive function of GlgA for glycogen synthesis in the absence of maltose metabolism. No glycogen was found in the ΔglgA and ΔglgA ΔmalP strains grown on glucose, but substantial amounts (0.18 and 1.0 mg/mg protein, respectively) were found when they were grown on maltodextrin. This demonstrates that the action of MalQ on maltose or maltodextrin can lead to the formation of glycogen and that MalP controls (inhibits) this pathway. In vitro, MalQ in the presence of GlgB (a branching enzyme) was able to form glycogen from maltose or linear maltodextrins. We propose a model of maltodextrin utilization for the formation of glycogen in the absence of glycogen synthase.
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