ArticleJournal of bacteriology2011
Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 54 citations in OpenAlex.
- Specialized shuttle proteins recognize Type IX secretion signals and target effectors to their final destinations in Flavobacterium johnsoniae.Communications biology · 2025Article
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- Gliding motility proteins GldJ and SprB contribute toJournal of bacteriology · 2024Article
- Surface colonization bymBio · 2024Article
- Differential gliding motility responses ofCurrent research in microbial sciences · 2024Article
- A journey with type IX secretion system effectors: selection, transport, processing and activities.Microbiology (Reading, England) · 2023Article
- A coevolution experiment betweenMicrobiology (Reading, England) · 2023Article
- Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery.Communications biology · 2023Article
- Transposon mutagenesis and genome sequencing identify two novel, tandem genes involved in the colony spreading ofFrontiers in cellular and infection microbiology · 2023Article
- Social Motility Assays of Flavobacterium johnsoniae.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Reversible mutations in gliding motility and virulence genes: A flexible and efficient phage defence mechanism in Flavobacterium psychrophilum.Environmental microbiology · 2022Article
- Dynamic proton-dependent motors power type IX secretion and gliding motility in Flavobacterium.PLoS biology · 2022Article
- MliR, a novel MerR-like regulator of iron homeostasis, impacts metabolism, membrane remodeling, and cell adhesion in the marine BacteroidetesFrontiers in microbiology · 2022Article
- The MonohemeMicrobiology spectrum · 2021Article
- Biofilm Spreading by the Adhesin-Dependent Gliding Motility ofInternational journal of molecular sciences · 2021Article
- Large-Scale Vortices with Dynamic Rotation Emerged from Monolayer Collective Motion of GlidingJournal of bacteriology · 2021Article
- Biofilm Spreading by the Adhesin-Dependent Gliding Motility ofInternational journal of molecular sciences · 2021Article
- Colony spreading of the gliding bacterium Flavobacterium johnsoniae in the absence of the motility adhesin SprB.Scientific reports · 2021Article
- A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.Science advances · 2020Article
- Global analysis of protein synthesis in Flavobacterium johnsoniae reveals the use of Kozak-like sequences in diverse bacteria.Nucleic acids research · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells of Flavobacterium johnsoniae exhibit rapid gliding motility over surfaces. Cell movement is thought to involve motor complexes comprised of Gld proteins that propel the cell surface adhesin SprB. The four distal genes of the sprB operon (sprC, sprD, sprB, and sprF) are required for normal motility and for formation of spreading colonies, but the roles of the remaining three genes (remF, remG, and fjoh_0982) are unclear. A gene deletion strategy was developed to determine whether these genes are involved in gliding. A spontaneous streptomycin-resistant rpsL mutant of F. johnsoniae was isolated. Introduction of wild-type rpsL on a plasmid restored streptomycin sensitivity, demonstrating that wild-type rpsL is dominant to the mutant allele. The gene deletion strategy employed a suicide vector carrying wild-type rpsL and used streptomycin for counterselection. This approach was used to delete the region spanning remF, remG, and fjoh_0982. The mutant cells formed spreading colonies, demonstrating that these genes are not required for normal motility. Analysis of the genome revealed a paralog of remF (remH) and a paralog of remG (remI). Deletion of remH and remI had no effect on motility of wild-type cells, but cells lacking remF and remH, or cells lacking remG and remI, formed nonspreading colonies. The motility defects resulting from the combination of mutations suggest that the paralogous proteins perform redundant functions in motility. The rpsL counterselection strategy allows construction of unmarked mutations to determine the functions of individual motility proteins or to analyze other aspects of F. johnsoniae physiology.
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