ArticleEukaryotic cell2011
Grx4 monothiol glutaredoxin is required for iron limitation-dependent inhibition of Fep1.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
- Monothiol glutaredoxins in microbial iron metabolism: From iron-sulfur cluster trafficking to iron-dependent regulation.Advances in microbial physiology · 2026Review
- The Monothiol Glutaredoxin Grx4 Influences Iron Homeostasis and Virulence inJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Coordinated regulation of iron metabolism in Cryptococcus neoformans by GATA and CCAAT transcription factors: connections with virulence.Current genetics · 2021Review
- Biophysical characterization of the complex between the iron-responsive transcription factor Fep1 and DNA.European biophysics journal : EBJ · 2021Article
- Iron-sulfur cluster biogenesis, trafficking, and signaling: Roles for CGFS glutaredoxins and BolA proteins.Biochimica et biophysica acta. Molecular cell research · 2021Review
- Molecular basis for the distinct functions of redox-active and FeS-transfering glutaredoxins.Nature communications · 2020Article
- Proteomic profiling of the monothiol glutaredoxin Grx3 reveals its global role in the regulation of iron dependent processes.PLoS genetics · 2020Article
- Iron-sulfur cluster signaling: The common thread in fungal iron regulation.Current opinion in chemical biology · 2020Review
- Yeast glutaredoxin, GRX4, functions as a glutathione S-transferase required for red ade pigment formation inJournal of biosciences · 2020Article
- The monothiol glutaredoxin GrxD is essential for sensing iron starvation in Aspergillus fumigatus.PLoS genetics · 2019Article
- A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX.PLoS pathogens · 2019Article
- The Monothiol Glutaredoxin Grx4 Regulates Iron Homeostasis and Virulence in Cryptococcus neoformans.mBio · 2018Article
- Iron-sensing is governed by mitochondrial, not by cytosolic iron-sulfur cluster biogenesis in Aspergillus fumigatus.Metallomics : integrated biometal science · 2018Article
- Schizosaccharomyces pombe Grx4 regulates the transcriptional repressor Php4 via [2Fe-2S] cluster binding.Metallomics : integrated biometal science · 2017Article
- Cytosolic iron chaperones: Proteins delivering iron cofactors in the cytosol of mammalian cells.The Journal of biological chemistry · 2017Review
- A Glutaredoxin·BolA Complex Serves as an Iron-Sulfur Cluster Chaperone for the Cytosolic Cluster Assembly Machinery.The Journal of biological chemistry · 2016Article
- Iron acquisition and oxidative stress response in aspergillus fumigatus.BMC systems biology · 2015Article
- Arabidopsis glutaredoxin S17 and its partner, the nuclear factor Y subunit C11/negative cofactor 2α, contribute to maintenance of the shoot apical meristem under long-day photoperiod.Plant physiology · 2015Article
- A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.PLoS genetics · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of iron transport genes in Schizosaccharomyces pombe is controlled by the Fep1 transcription factor. When iron levels exceed those needed by the cells, Fep1 represses iron transport genes. In contrast, Fep1 is unable to bind chromatin under low-iron conditions, and that results in activation of genes involved in iron acquisition. Studies of fungi have revealed that monothiol glutaredoxins are required to inhibit iron-dependent transcription factors in response to high levels of iron. Here, we show that the monothiol glutaredoxin Grx4 plays an important role in the negative regulation of Fep1 activity in response to iron deficiency. Deletion of the grx4(+) gene led to constitutive promoter occupancy by Fep1 and caused an invariable repression of iron transport genes. We found that Grx4 and Fep1 physically interact with each other. Grx4 contains an N-terminal thioredoxin (TRX)-like domain and a C-terminal glutaredoxin (GRX)-like domain. Deletion mapping analysis revealed that the TRX domain interacts strongly and constitutively with the C-terminal region of Fep1. As opposed to the TRX domain, the GRX domain associates weakly and in an iron-dependent manner with the N-terminal region of Fep1. Further analysis showed that Cys35 of Grx4 is required for the interaction between the Fep1 C terminus and the TRX domain, whereas Grx4 Cys172 is necessary for the association between the Fep1 N terminus and the GRX domain. Our results describe the first example of a monothiol glutaredoxin that acts as an inhibitory partner for an iron-regulated transcription factor under conditions of low iron levels.
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