ArticleThe Journal of urology2011
ZEB1 is estrogen responsive in vitro in human foreskin cells and is over expressed in penile skin in patients with severe hypospadias.
Article in The Journal of urology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Downregulated androgen receptor expression in preputial tissues of children with hypospadias.Frontiers in pediatrics · 2025Article
- Langerhans cells in hypospadias: an analysis of Langerin (CD207) and HLA-DR on epidermal sheets and full thickness skin sections.BMC urology · 2019Article
- Tissue-specific roles of Fgfr2 in development of the external genitalia.Development (Cambridge, England) · 2015Article
- Current understanding of hypospadias: relevance of animal models.Nature reviews. Urology · 2015Review
- The Genetic and Environmental Factors Underlying Hypospadias.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2015Review
- Androgen receptor is overexpressed in boys with severe hypospadias, and ZEB1 regulates androgen receptor expression in human foreskin cells.Pediatric research · 2012Article
- Expression of estrogen receptor alpha and beta is decreased in hypospadias.The Journal of urology · 2012Article
- Identification of novel low-dose bisphenol a targets in human foreskin fibroblast cells derived from hypospadias patients.PloS one · 2012Article
- Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor.International journal of endocrinology · 2011Article
- Androgen insensitivity syndrome: a review.Archives of endocrinology and metabolismReview
- Androgen Receptor Expression in Hypospadias.Journal of Indian Association of Pediatric SurgeonsArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeWe determined the effect of estrogen on ZEB1 in vitro and tested the hypothesis that ZEB1 is over expressed in the penile skin of subjects with hypospadias. MATERIALS AND
methodsHs68 cells, a fibroblast cell line derived from human foreskin, were exposed to 0, 1, 10 and 100 nM estrogen, and the expression level of ZEB1 was assessed using reverse transcription real-time polymerase chain reaction, Western blot and immunocytochemical analysis. Next, preputial skin was prospectively collected from case and control subjects at hypospadias repair (37 cases) and circumcision (11). Hypospadias was classified as severe (13 cases) or mild (24) based on the position of the urethral meatus. ZEB1 expression was quantified using reverse transcription real-time polymerase chain reaction, Western blot and immunohistochemical analysis.
resultsEstrogen increased ZEB1 expression at the mRNA and protein levels in Hs68 cells in a concentration dependent fashion (p <0.01). Subjects with severe hypospadias had significantly higher ZEB1 mRNA levels and protein expression compared to controls or subjects with mild hypospadias (both p <0.01). Subjects with severe hypospadias had increased expression of ZEB1 in the basal layers of the preputial epidermis.
conclusionsEstrogen increases ZEB1 expression in a human foreskin fibroblast cell line in vitro. Furthermore, ZEB1 is significantly over expressed in the penile skin of subjects with severe hypospadias. We propose that ZEB1 overexpression may contribute to development of hypospadias and may mediate the effect of estrogen on developing external male genitalia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.