ArticleHeart rhythm2011
Funny current channel HCN4 delineates the developing cardiac conduction system in chicken heart.
Article in Heart rhythm, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- The Neglected Internodal Tract-A Cardiac Conduction System Structure Homologous to the Development and Regulation of the Sinoatrial Node.Reviews in cardiovascular medicine · 2025Review
- Development of autonomic innervation at the venous pole of the heart: bridging the gap from mice to human.Journal of translational medicine · 2025Article
- Human heart-forming organoids recapitulate early heart and foregut development.Nature biotechnology · 2021Article
- Assembly of the Cardiac Pacemaking Complex: Electrogenic Principles of Sinoatrial Node Morphogenesis.Journal of cardiovascular development and disease · 2021Review
- Development of the Cardiac Conduction System.Cold Spring Harbor perspectives in biology · 2020Review
- HCN2 Channel-Induced Rescue of Brain TeratogenesisFrontiers in cellular neuroscience · 2020Article
- Development and evolution of the metazoan heart.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Review
- Transcriptional regulation of the cardiac conduction system.Nature reviews. Cardiology · 2018Review
- HCN2 Rescues brain defects by enforcing endogenous voltage pre-patterns.Nature communications · 2018Article
- HCN4 ion channel function is required for early events that regulate anatomical left-right patterning in a nodal and lefty asymmetric gene expression-independent manner.Biology open · 2017Article
- Coordinating heart morphogenesis: A novel role for hyperpolarization-activated cyclic nucleotide-gated (HCN) channels during cardiogenesis inCommunicative & integrative biology · 2017Article
- Same-Single-Cell Analysis of Pacemaker-Specific Markers in Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Subtypes Classified by Electrophysiology.Stem cells (Dayton, Ohio) · 2016Article
- Mechanisms underlying the cardiac pacemaker: the role of SK4 calcium-activated potassium channels.Acta pharmacologica Sinica · 2016Review
- The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?Journal of cellular and molecular medicine · 2015Article
- Does the dorsal mesenchymal protrusion act as a temporary pacemaker during heart development?The Journal of biological chemistry · 2015Article
- Right Ventricular Outflow Tract Arrhythmias: Benign Or Early Stage Arrhythmogenic Right Ventricular Cardiomyopathy/Dysplasia?Journal of atrial fibrillation · 2014Review
- Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes.Molecular and cellular biochemistry · 2013Article
- A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells.Nature cell biology · 2013Article
- Radiofrequency catheter ablation of idiopathic right ventricular outflow tract arrhythmias.Indian pacing and electrophysiology journal · 2013Article
- Spatiotemporal regulation of an Hcn4 enhancer defines a role for Mef2c and HDACs in cardiac electrical patterning.Developmental biology · 2013Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) in the mouse is expressed in the developing cardiac conduction system (CCS). In the sinoatrial node (SAN), HCN4 is the predominant isoform responsible for the funny current. To date, no data are available on HCN4 expression during chicken CCS development.
objectiveThe purpose of this study was to provide the full-length sequence of Hcn4 and describe its expression pattern during development in relation to the CCS in the chicken embryo.
methodsHcn4 RNA expression was studied by in situ hybridization in sequential chick developmental stages (HH11-HH35) and immunohistochemical staining was conducted for the myocardial protein cardiac troponin I and the cardiac transcription factor Nkx2.5.
resultsWe obtained the full-length sequence of Hcn4 in chick. Hcn4 expression was observed early in development in the primary heart tube. At later stages, expression became restricted to transitional zones flanked by working myocardium, comprising the sinus venosus myocardium where the SAN develops, the atrioventricular canal myocardium, the primary fold (a myocardial zone between the developing ventricles), and the developing outflow tract. Further in development, Hcn4 expression was restricted to the SAN, the atrioventricular node, the common bundle, the bundle branches, and the internodal and atrioventricular ring myocardium.
conclusionWe have identified Hcn4 as a marker of the developing CCS in the chick. The primary heart tube expresses Hcn4, which is later restricted to the transitional zones and eventually the elements of the mature CCS. Furthermore, we hypothesize that expression patterns during development may delineate potential arrhythmogenic sites in the adult heart.
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