Evidence map›Paper›PMID 21420447›Full record

ArticleJournal of microbiological methods2011

Sensitivity of Francisella tularensis to ultrapure water and deoxycholate: implications for bacterial intracellular growth assay in macrophages.

Sabina Chalabaev, Christine A Anderson, Andrew B Onderdonk, Dennis L Kasper

Abstract read
In one paragraph

Article in Journal of microbiological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Contributions of TolC Orthologs toInfection and immunity · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sabina ChalabaevDepartment of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, United States.
Christine A Anderson
Andrew B Onderdonk
Dennis L Kasper
Brigham and Women's Hospital · USHarvard University · US

Funding

Treatments for Nipah Virus InfectionU54AI057159 · NIAID · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WHELAN, SEAN PJ · 2003 to 2013
$113.7M
NIAID NIH HHS AI057159NIAID NIH HHS U54 AI057159
6 · The paper itself

Abstract

The ability of Francisella tularensis to replicate in macrophages is critical for its pathogenesis, therefore intracellular growth assays are important tools for assessing virulence. We show that two lysis solutions commonly used in these assays, deionized water and deoxycholate in PBS, lead to highly inaccurate measurements of intracellular bacterial survival.

Indexed as

Anti-Bacterial AgentsDeoxycholic AcidFrancisella tularensisMacrophagesMicrobial Sensitivity TestsMicrobial ViabilityWaterAnti-Bacterial AgentsDeoxycholic AcidWater

Identifiers

PMID21420447
PMCPMC3098563
OpenAlexW1965601614

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.