ArticleMolecular and biochemical parasitology
A Gateway® compatible vector for gene silencing in bloodstream form Trypanosoma brucei.
Article in Molecular and biochemical parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation iniScience · 2020Article
- Pseudouridines on Trypanosoma brucei spliceosomal small nuclear RNAs and their implication for RNA and protein interactions.Nucleic acids research · 2019Article
- Small nucleolar RNAs controlling rRNA processing in Trypanosoma brucei.Nucleic acids research · 2019Article
- A dual regulatory circuit consisting of S-adenosylmethionine decarboxylase protein and its reaction product controls expression of the paralogous activator prozyme in Trypanosoma brucei.PLoS pathogens · 2018Article
- Characterization of the novel mitochondrial genome replication factor MiRF172 inJournal of cell science · 2018Article
- TelAP1 links telomere complexes with developmental expression site silencing in African trypanosomes.Nucleic acids research · 2018Article
- Molecular model of the mitochondrial genome segregation machinery inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Simplified inducible system for Trypanosoma brucei.PloS one · 2018Article
- Pyrimidine Salvage Enzymes Are Essential for De Novo Biosynthesis of Deoxypyrimidine Nucleotides in Trypanosoma brucei.PLoS pathogens · 2016Article
- Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei.Molecular biology of the cell · 2015Article
- Deoxyhypusine Modification of Eukaryotic Translation Initiation Factor 5A (eIF5A) Is Essential for Trypanosoma brucei Growth and for Expression of Polyprolyl-containing Proteins.The Journal of biological chemistry · 2015Article
- A new strategy of RNA interference that targets heterologous sequences reveals CITFA1 as an essential component of class I transcription factor A in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Genetic validation of aminoacyl-tRNA synthetases as drug targets in Trypanosoma brucei.Eukaryotic cell · 2014Article
- On the extent and role of the small proteome in the parasitic eukaryote Trypanosoma brucei.BMC biology · 2014Article
- A multiple aminoacyl-tRNA synthetase complex that enhances tRNA-aminoacylation in African trypanosomes.Molecular and cellular biology · 2013Article
- Product feedback regulation implicated in translational control of the Trypanosoma brucei S-adenosylmethionine decarboxylase regulatory subunit prozyme.Molecular microbiology · 2013Article
- Comparative proteomics of two life cycle stages of stable isotope-labeled Trypanosoma brucei reveals novel components of the parasite's host adaptation machinery.Molecular & cellular proteomics : MCP · 2013Article
- A single-cloning-step procedure for the generation of RNAi plasmids producing long stem-loop RNA.Molecular and biochemical parasitology · 2012Article
- RNA interference in protozoan parasites: achievements and challenges.Eukaryotic cell · 2011Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference is the most rapid method for generation of conditional knockdown mutants in Trypanosoma brucei. The dual T7 promoter (pZJM) and the stem-loop vectors have been widely used to generate stable inducible RNAi cell lines with the latter providing tighter regulatory control. However, the steps for cloning stem-loop constructs are cumbersome requiring either multiple cloning steps or multi-fragment ligation reactions. We report the development of a vector (pTrypRNAiGate) derived from pLEW100 that utilizes the Gateway® recombination system to facilitate easy production of hairpin RNA constructs. This approach allows the final stem-loop RNAi construct to be generated from a single cloning step of the PCR-derived gene fragment followed by an in vitro recombination reaction. The new vector facilitates high-throughput applications for gene silencing and provides a tool for functional genomics in T. brucei.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.