ArticleMolecular cell2011
Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease.
Article in Molecular cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 168 citations in OpenAlex.
- Molecular basis of plant DCL4 action that outcompetes DCL2.Nature plants · 2026Article
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- Antagonism of RNA silencing in the yellow fever mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Biochemical and structural basis of Dicer helicase function unveiled by resurrecting ancient proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- In vivo Dicer-2 interactome during viral infection reveals novel pro and antiviral factors in Drosophila melanogaster.PLoS pathogens · 2025Article
- The PAZ domain ofRNA (New York, N.Y.) · 2025Article
- Cell-Autonomous and Non-Cell-Autonomous Antiviral Immunity via siRNA-Directed RNAi inImmune discovery · 2025Article
- DsRNA as pathogen-associated molecular pattern in innate immunity and multiple functions of the RNAi machinery complicate the use of RNAi in pest control.Frontiers in insect science · 2025Review
- The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding.BMC biology · 2024Article
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- Review
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- Cellular functions of eukaryotic RNA helicases and their links to human diseases.Nature reviews. Molecular cell biology · 2023Review
- Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.Nature communications · 2023Article
- Dicer structure and function: conserved and evolving features.EMBO reports · 2023Review
- Exosomes induce neurogenesis of pluripotent P19 cells.Stem cell reviews and reports · 2023Article
- Ancestral protein reconstruction reveals evolutionary events governing variation in Dicer helicase function.eLife · 2023Article
- Structural and functional basis of mammalian microRNA biogenesis by Dicer.Molecular cell · 2022Article
- Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.Molecular cell · 2022Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Drosophila Dicer-2 generates small interfering RNAs (siRNAs) from long double-stranded RNA (dsRNA), whereas Dicer-1 produces microRNAs (miRNAs) from pre-miRNA. What makes the two Dicers specific for their biological substrates? We find that purified Dicer-2 can efficiently cleave pre-miRNA, but that inorganic phosphate and the Dicer-2 partner protein R2D2 inhibit pre-miRNA cleavage. Dicer-2 contains C-terminal RNase III domains that mediate RNA cleavage and an N-terminal helicase motif, whose function is unclear. We show that Dicer-2 is a dsRNA-stimulated ATPase that hydrolyzes ATP to ADP; ATP hydrolysis is required for Dicer-2 to process long dsRNA, but not pre-miRNA. Wild-type Dicer-2, but not a mutant defective in ATP hydrolysis, can generate siRNAs faster than it can dissociate from a long dsRNA substrate. We propose that the Dicer-2 helicase domain uses ATP to generate many siRNAs from a single molecule of dsRNA before dissociating from its substrate.
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