ArticleBiochemical and biophysical research communications2011
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.
Article in Biochemical and biophysical research communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 76 citations in OpenAlex.
- How advances in cryo-electron tomography have contributed to our current view of bacterial cell biology.Journal of structural biology: X · 2022Article
- The wall-less bacteriumiScience · 2021Article
- Challenges and triumphs in cryo-electron tomography.iScience · 2021Review
- Superstructure formation by RodZ hexamers of Shigella sonnei maintains the rod shape of bacilli.PloS one · 2020Article
- A collection of yeast cellular electron cryotomography data.GigaScience · 2019Article
- In Cellulo Synthesis of Proteins Containing a Fluorescent Oxazole Amino Acid.Journal of the American Chemical Society · 2019Article
- Cryo-SOFI enabling low-dose super-resolution correlative light and electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.Chemical reviews · 2018Review
- Polar localization of MreB actin is inhibited by anionic phospholipids in the rod-shaped bacterium Escherichia coli.Current genetics · 2017Review
- Uncharacterized Bacterial Structures Revealed by Electron Cryotomography.Journal of bacteriology · 2017Article
- The Physiology of Phagocytosis in the Context of Mitochondrial Origin.Microbiology and molecular biology reviews : MMBR · 2017Review
- Super-Resolution Microscopy and Single-Protein Tracking in Live Bacteria Using a Genetically Encoded, Photostable Fluoromodule.Current protocols in cell biology · 2017Article
- Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium "Candidatus Pelagibacter ubique".Applied and environmental microbiology · 2017Article
- Correlated fluorescence microscopy and cryo-electron tomography of virus-infected or transfected mammalian cells.Nature protocols · 2017Article
- A new view into prokaryotic cell biology from electron cryotomography.Nature reviews. Microbiology · 2016Review
- Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions.Scientific reports · 2015Article
- The small G-protein MglA connects to the MreB actin cytoskeleton at bacterial focal adhesions.The Journal of cell biology · 2015Article
- Coarse-grained simulations of bacterial cell wall growth reveal that local coordination alone can be sufficient to maintain rod shape.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Bacterial Filament Systems: Toward Understanding Their Emergent Behavior and Cellular Functions.The Journal of biological chemistry · 2015Review
- Mechanisms of bacterial morphogenesis: evolutionary cell biology approaches provide new insights.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
How rod-shaped bacteria form and maintain their shape is an important question in bacterial cell biology. Results from fluorescent light microscopy have led many to believe that the actin homolog MreB and a number of other proteins form long helical filaments along the inner membrane of the cell. Here we show using electron cryotomography of six different rod-shaped bacterial species, at macromolecular resolution, that no long (> 80 nm) helical filaments exist near or along either surface of the inner membrane. We also use correlated cryo-fluorescent light microscopy (cryo-fLM) and electron cryo-tomography (ECT) to identify cytoplasmic bundles of MreB, showing that MreB filaments are detectable by ECT. In light of these results, the structure and function of MreB must be reconsidered: instead of acting as a large, rigid scaffold that localizes cell-wall synthetic machinery, moving MreB complexes may apply tension to growing peptidoglycan strands to ensure their orderly, linear insertion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.