ArticleMicrobial biotechnology2011
Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440.
Article in Microbial biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 83 citations in OpenAlex.
- Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates.Microbial biotechnology · 2026Article
- Engineering of bacteria towards programmed autolysis: why, how, and when?Microbial cell factories · 2024Review
- Recent Trends in the Production and Recovery of Bioplastics Using Polyhydroxyalkanoates Copolymers.Microorganisms · 2024Review
- Manipulating Microbial Cell Morphology for the Sustainable Production of Biopolymers.Polymers · 2024Review
- Assessing the Orthogonality of Phage-Encoded RNA Polymerases for Tailored Synthetic Biology Applications inInternational journal of molecular sciences · 2023Article
- Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery.Microbial biotechnology · 2023Review
- Reprogramming microbial populations using a programmed lysis system to improve chemical production.Nature communications · 2021Article
- Separation and purification of plant terpenoids from biotransformation.Engineering in life sciences · 2021Review
- A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution.ACS synthetic biology · 2021Article
- Post-Transcriptional Control in the Regulation of Polyhydroxyalkanoates Synthesis.Life (Basel, Switzerland) · 2021Review
- Biological Approaches in Polyhydroxyalkanoates Recovery.Current microbiology · 2021Review
- Recovery of Polyhydroxyalkanoates From Single and Mixed Microbial Cultures: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Exploring the synthetic biology potential of bacteriophages for engineering non-model bacteria.Nature communications · 2020Review
- From Organic Wastes to Bioplastics: Feasibility of Nonsterile Poly(3-hydroxybutyrate) Production byACS omega · 2020Article
- Engineering the Osmotic State ofFrontiers in bioengineering and biotechnology · 2020Article
- Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain.Microbial biotechnology · 2019Article
- The XylS/Pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering.Microbial biotechnology · 2017Review
- A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.Scientific reports · 2017Article
- Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production.Bioengineering (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of PHA granules to the extracellular medium. The new autolytic cell disruption system is based on two simultaneous strategies: the coordinated action of two proteins from the pneumococcal bacteriophage EJ-1, an endolysin (Ejl) and a holin (Ejh), and the mutation of the tolB gene, which exhibits alterations in outer membrane integrity that induce lysis hypersensitivity. The ejl and ejh coding genes were expressed under a XylS/Pm monocopy expression system inserted into the chromosome of the tolB mutant strain, in the presence of 3-methylbenzoate as inducer molecule. Our results demonstrate that the intracellular presence of PHA granules confers resistance to cell envelope. Conditions to control the cell autolysis in P. putida BXHL in terms of optimal fermentation, PHA content and PHA recovery have been set up by exploring the sensitivity to detergents, chelating agents and wet biomass solubility in organic solvents such as ethyl acetate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.