ArticleMicrocirculation (New York, N.Y. : 1994)2011
Intravital macrozoom imaging and automated analysis of endothelial cell calcium signals coincident with arteriolar dilation in Cx40(BAC) -GCaMP2 transgenic mice.
Article in Microcirculation (New York, N.Y. : 1994), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- Computer-assisted image analysis of agonist-mediated microvascular constriction response in mouse cremaster muscle.PloS one · 2022Article
- Microvascular dysfunction and kidney disease: Challenges and opportunities?Microcirculation (New York, N.Y. : 1994) · 2021Review
- Calcium Signal Profiles in Vascular Endothelium from Cdh5-GCaMP8 and Cx40-GCaMP2 Mice.Journal of vascular research · 2021Article
- Calcium signals that determine vascular resistance.Wiley interdisciplinary reviews. Systems biology and medicine · 2019Review
- The Endothelium Solves Problems That Endothelial Cells Do Not Know Exist.Trends in pharmacological sciences · 2017Review
- Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery.Journal of visualized experiments : JoVE · 2015Article
- Optogenetic sensors and effectors: CHROMus-the Cornell Heart Lung Blood Institute Resource for Optogenetic Mouse Signaling.Frontiers in physiology · 2014Review
- Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.Microcirculation (New York, N.Y. : 1994) · 2013Article
- Ageing alters perivascular nerve function of mouse mesenteric arteries in vivo.The Journal of physiology · 2013Article
- Visualizing calcium responses to acetylcholine convection along endothelium of arteriolar networks in Cx40BAC-GCaMP2 transgenic mice.American journal of physiology. Heart and circulatory physiology · 2011Article
- Hemorheological implications of perfluorocarbon based oxygen carrier interaction with colloid plasma expanders and blood.Biotechnology progressArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
objectiveCalcium signaling is integral to endothelium-dependent vasodilation. Our goal was to develop methods enabling automated analyses for accurately and objectively determining the dynamic relationship between EC Ca(2+) responses and arteriolar diameter in vivo.
methodsUser-friendly software (DiaFluor) written in LabView was applied to images acquired at 15fps with a custom macrozoom intravital microscope to evaluate changes in EC Ca(2+) concomitant with arteriolar diameter. Transgenic Cx40(BAC) -GCaMP2 mice expressing a fluorescent Ca(2+) indicator molecule in arteriolar ECs enabled resolution of EC Ca(2+) signaling in response to ACh microiontophoresis (500nA, 100-1000msec pulse) from a micropipette (1μm tip) positioned adjacent to an arteriole in the superfused cremaster muscle preparation.
resultsA 100-msec pulse of ACh (1M) had little effect on EC Ca(2+) or arteriolar diameter. As pulse duration increased, vasodilation increased with fluorescence intensity (p<0.01). Based upon fluorescence responses (F/F(o)), the effective diffusion distance of ACh along arterioles increased from ∼100μm (250msec pulse) to ∼200μm (1000msec pulse) with a peak velocity of ∼150μm/sec.
conclusionsThe novel imaging and software presented here are the first to enable automated simultaneous evaluation of EC Ca(2+) signaling and endothelium-dependent vasodilation in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.