ArticleMicrocirculation (New York, N.Y. : 1994)2011
Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting.
Article in Microcirculation (New York, N.Y. : 1994), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
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- Imaging of the immune system - towards a subcellular and molecular understanding.Journal of cell science · 2020Review
- High-Affinity Bent βCell reports · 2019Article
- Dual catenin loss in murine liver causes tight junctional deregulation and progressive intrahepatic cholestasis.Hepatology (Baltimore, Md.) · 2018Article
- Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix.Journal of visualized experiments : JoVE · 2015Article
- Genetic deletion of platelet glycoprotein Ib alpha but not its extracellular domain protects from atherosclerosis.Thrombosis and haemostasis · 2014Article
- 'Slings' enable neutrophil rolling at high shear.Nature · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveNeutrophil recruitment to sites of inflammation involves P-selectin-dependent rolling. qDF is a useful tool to visualize the topography of the neutrophil footprint as it interacts with the substrate. However, elucidating the role of specific proteins in addition to topography requires simultaneous visualization of two fluorochromes.
methodsTo validate DqDF, mouse neutrophils were labeled with the membrane dyes DiO and DiI and perfused into microchannels coated with P-selectin-Fc. Footprints of rolling neutrophils were recorded as two separate images, one for each fluorochrome. To assess the localization of the cytoskeletal protein paxillin, we applied DqDF to DiO-stained neutrophils of mice expressing an mCherry-paxillin fusion protein.
resultsThe footprint topographies obtained from DiO and DiI in the plasma membrane were identical. The z-coordinates of the microvilli tips obtained with the two fluorochromes in the footprint were also identical. Paxillin was found to be localized to some, but not all ridges in the neutrophil footprint.
conclusionsOur data suggest that the spectral properties of the fluorochrome do not affect the results. DqDF will be useful for simultaneous visualization of two fluorochromes in the footprint of rolling cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.