ArticleClinical orthopaedics and related research2011
Molecular techniques to detect biofilm bacteria in long bone nonunion: a case report.
Article in Clinical orthopaedics and related research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Establishment and application of TSDPSO-SVM model combined with multi-dimensional feature fusion method in the identification of fracture-related infection.Scientific reports · 2023Article
- Fluorescence In Situ Hybridization as Diagnostic Tool for Implant-associated Infections: A Pilot Study on Added Value.Plastic and reconstructive surgery. Global open · 2023Article
- High prevalence of bacteria in clinically aseptic non-unions of the tibia and the femur in tissue biopsies.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2020Article
- Malnutrition and Fracture Healing: Are Specific Deficiencies in Amino Acids Important in Nonunion Development?Nutrients · 2018Review
- [The significance of biofilm for the treatment of infections in orthopedic surgery : 2017 Update].Der Unfallchirurg · 2017Review
- Cytotoxicity of a new antimicrobial coating for surgical screws: an in vivo study.Singapore medical journal · 2017Article
- Polymerase Chain Reaction-Electrospray-Time-of-Flight Mass Spectrometry Versus Culture for Bacterial Detection in Septic Arthritis and Osteoarthritis.Genetic testing and molecular biomarkers · 2016Article
- D-amino acid inhibits biofilm but not new bone formation in an ovine model.Clinical orthopaedics and related research · 2015Article
- Innovative strategies for the management of long bone infection: a review of the Masquelet technique.Patient safety in surgery · 2015Review
- Propionibacterium acnes and Staphylococcus lugdunensis cause pyogenic osteomyelitis in an intramedullary nail model in rabbits.Journal of clinical microbiology · 2014Article
- Optimal culture incubation time in orthopedic device-associated infections: a retrospective analysis of prolonged 14-day incubation.Journal of clinical microbiology · 2014Article
- Composition and properties of silver-containing calcium carbonate-calcium phosphate bone cement.Journal of materials science. Materials in medicine · 2013Article
- Staphylococcus aureus biofilms decrease osteoblast viability, inhibits osteogenic differentiation, and increases bone resorption in vitro.BMC musculoskeletal disorders · 2013Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundBiofilms cause chronic infections including those associated with orthopaedic hardware. The only methods that are Food and Drug Administration-approved for detecting and identifying bacterial infections are cultures and selected DNA-based polymerase chain reaction methods that detect only specific pathogens (eg, methicillin-resistant Staphylococcus aureus). New DNA-based technologies enable the detection and identification of all bacteria present in a sample and to determine the antibiotic sensitivities of the organisms. CASE DESCRIPTION: A 34-year-old man sustained an open tibia fracture. He experienced 3 years of delayed healing and episodic pain. In addition to his initial treatment, he underwent three additional surgeries to achieve fracture healing. During the last two procedures, cultures were taken and samples were tested with the IBIS T5000 and fluorescence in situ hybridization (FISH). In both cases, the cultures were negative, but the IBIS and FISH confirmed the presence of a biofilm within the tibial canal. LITERATURE REVIEW: Examinations of tissues from biofilm infections, by DNA-based molecular methods and by direct microscopy, have often found bacteria present despite negative cultures. Infections associated with orthopaedic hardware may be caused by bacteria living in biofilms, and these biofilm organisms are particularly difficult to detect by routine culture methods. PURPOSES AND CLINICAL RELEVANCE: Rapid DNA-based detection methods represent a potentially clinically useful tool in the detection of bacterial biofilms. The sensitivity and clinical impact of the technology has yet to be established.
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