ArticleMolecular biotechnology2011
Reference gene selection for quantitative real-time PCR in Chrysanthemum subjected to biotic and abiotic stress.
Article in Molecular biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 146 citations in OpenAlex.
- Genome-Wide Analysis of the Hsf Family and Functional Characterization ofPlants (Basel, Switzerland) · 2026Article
- High-Blue/Low-Red Mixed Light Modulates Photoperiodic Flowering in Chrysanthemum via Photoreceptor and Sugar Pathways.Plants (Basel, Switzerland) · 2025Article
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- Both the Positioned Supplemental or Night-Interruptional Blue Light and the Age of Leaves (or Tissues) Are Important for Flowering and Vegetative Growth in Chrysanthemum.Plants (Basel, Switzerland) · 2024Article
- Systematic Identification and Characterization ofInternational journal of molecular sciences · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Comparative Analysis of Physical and Chemical Mutagenesis in Chrysanthemum cv. 'Candid': Assessing Genetic Variation and Breeding Potential.ACS omega · 2023Article
- CmWRKY6-1-CmWRKY15-like transcriptional cascade negatively regulates the resistance toHorticulture research · 2023Article
- Potential pathways and genes expressed in Chrysanthemum in response to early fusarium oxysporum infection.BMC plant biology · 2023Article
- Overexpression ofInternational journal of molecular sciences · 2023Article
- Rhamnosyltransferases involved in the biosynthesis of flavone rutinosides in Chrysanthemum species.Plant physiology · 2022Article
- Blue Light Supplemented at Intervals in Long-Day Conditions Intervenes in Photoperiodic Flowering, Photosynthesis, and Antioxidant Properties in Chrysanthemums.Antioxidants (Basel, Switzerland) · 2022Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
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- The BBX geneHorticulture research · 2022Article
- The flowering of SDP chrysanthemum in response to intensity of supplemental or night-interruptional blue light is modulated by both photosynthetic carbon assimilation and photoreceptor-mediated regulation.Frontiers in plant science · 2022Article
- Evaluation of Angelica decursiva reference genes under various stimuli for RT-qPCR data normalization.Scientific reports · 2021Article
- Overexpression of the CmJAZ1-like gene delays flowering in Chrysanthemum morifolium.Horticulture research · 2021Article
- Two Cyc2CL transcripts (Cyc2CL-1 and Cyc2CL-2) may play key roles in the petal and stamen development of ray florets in chrysanthemum.BMC plant biology · 2021Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (RT-qPCR) is a reliable method for assessing gene expression, provided that suitable reference genes are included to normalize the data. The stability of expression of eight potential reference genes, namely, tubulin (alpha-2,4 tubulin), actin, EF1 α (elongation factor 1 α), UBC (ubiquitin C), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), psaA (photosynthesis-related plastid gene representing photosystem I), PP2Acs (catalytic subunit of protein phosphatase 2A), and PGK (phosphoglycerate kinase), was assessed in chrysanthemum plants subjected to aphid infestation, heat stress or waterlogging stress using geNorm software. The widely used reference gene EF1 α performed well for aphid infested plants but poorly for waterlogged ones. The catalytic subunit of protein phosphatase 2A (PP2Acs) was the best performing one during heat and waterlogging stress, but was the worst during aphid infestation. The commonly used reference gene actin was generally the least stable of the set. No single gene was suitable for normalization on its own. The choice of reference gene(s) is an important factor in gene expression studies based on RT-qPCR.
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