ArticleVirology journal2011
Specific, simple and rapid detection of porcine circovirus type 2 using the loop-mediated isothermal amplification method.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- MicroRNAs: exploring their role in farm animal disease and mycotoxin challenges.Frontiers in veterinary science · 2024Review
- Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of African Swine Fever Virus, Porcine Circovirus 2, and Pseudorabies Virus in East China from 2020 to 2022.Veterinary sciences · 2023Article
- Visual and Rapid Detection of Porcine Epidemic Diarrhea Virus (PEDV) Using Reverse Transcription Loop-Mediated Isothermal Amplification Method.Animals : an open access journal from MDPI · 2022Article
- Visual and label-free ASFV and PCV2 detection by CRISPR-Cas12a combined with G-quadruplex.Frontiers in veterinary science · 2022Article
- Sensitive detection of porcine circovirus 3 by droplet digital PCR.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Development of a real time reverse transcription loop-mediated isothermal amplification method (RT-LAMP) for detection of a novel swine acute diarrhea syndrome coronavirus (SADS-CoV).Journal of virological methods · 2018Article
- Identification and characterization of microRNA in the lung tissue of pigs with different susceptibilities to PCV2 infection.Veterinary research · 2018Article
- Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2.BioMed research international · 2017Article
- A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification (LAMP).Frontiers in microbiology · 2014Article
- Reverse transcription loop-mediated isothermal amplification for rapid detection of the newly emerged poultry Flavivirus.Folia microbiologica · 2013Article
- Development of a loop-mediated isothermal amplification method to rapidly detect porcine circovirus genotypes 2a and 2b.Virology journal · 2012Article
- Molecular techniques for pathogen identification and fungus detection in the environment.IMA fungus · 2011Article
- Simultaneous detection and differentiation of dengue virus serotypes 1-4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay.Virology journal · 2011Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPorcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS), and porcine dermatitis and nephropathy syndrome (PDNS). It has caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS.
resultsA loop-mediated isothermal amplification (LAMP) assay was used to detect PCV2 in this study. Three pairs of primers were specially designed for recognizing eight distinct sequences of the ORF2 gene. This gene lies in the PCV2 virus genome sequence, and encodes the Rep protein that is involved in virus replication. Time and temperature conditions for amplification of PCV2 genes were optimized to be 55 min at 59°C. The analysis of clinical samples indicated that the LAMP method was highly sensitive. The detection limit for PCV2 by the LAMP assay was 10 copies, whereas the limit by conventional PCR was 1000 copies. The assay did not cross-react with PCV1, porcine reproductive and respiratory syndrome virus, porcine epidemic diarrhea virus, transmissible gastroenteritis of pigs virus or rotavirus. When 110 samples were tested using the established LAMP system, 95 were detected as positive.
conclusionThe newly developed LAMP detection method for PCV2 was more specific, sensitive, rapid and simple than before. It complements and extends previous methods for PCV2 detection and provides an alternative approach for detection of PCV2.
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