ArticleJournal of cellular physiology2011
High capacity Na+/H+ exchange activity in mineralizing osteoblasts.
Article in Journal of cellular physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Spectrum and functions of ion channels and transporters in osteoclasts.Bone research · 2026Review
- Energy-Dependent Phosphate and Acid Transport for Bone Formation and Resorption.Journal of cellular biochemistry · 2025Review
- Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis.Nature cell biology · 2025Article
- Atlantoaxial Instability due to Os Odontoideum in a Child with Christianson Syndrome.Molecular syndromology · 2024Article
- Chloride/proton antiporters ClC3 and ClC5 support bone formation in mice.Bone reports · 2024Article
- The ecto-nucleotide pyrophosphatase/phosphodiesterase 2 promotes early osteoblast differentiation and mineralization in stromal stem cells.American journal of physiology. Cell physiology · 2024Article
- Epithelial-like transport of mineral distinguishes bone formation from other connective tissues.Journal of cellular biochemistry · 2023Review
- Age-related decline in bone mineral transport and bone matrix proteins in osteoblasts from stromal stem cells.American journal of physiology. Cell physiology · 2023Article
- Molecular mechanisms, physiological roles, and therapeutic implications of ion fluxes in bone cells: Emphasis on the cation-ClJournal of cellular physiology · 2022Review
- Donor Splice Site Variant inMedicina (Kaunas, Lithuania) · 2022Article
- Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform.Communications biology · 2022Article
- An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Cellular bicarbonate accumulation and vesicular proton transport promote calcification in the sea urchin larva.Proceedings. Biological sciences · 2020Article
- Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport.American journal of physiology. Cell physiology · 2020Review
- pH and male fertility: making sense on pH homeodynamics throughout the male reproductive tract.Cellular and molecular life sciences : CMLS · 2019Review
- Support of bone mineral deposition by regulation of pH.American journal of physiology. Cell physiology · 2018Article
- Article
- Hypoxia-induced mobilization of NHE6 to the plasma membrane triggers endosome hyperacidification and chemoresistance.Nature communications · 2017Article
- Osteoblast Differentiation and Bone Matrix Formation In Vivo and In Vitro.Tissue engineering. Part B, Reviews · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Osteoblasts synthesize bone in polarized groups of cells sealed by tight junctions. Large amounts of acid are produced as bone mineral is precipitated. We addressed the mechanism by which cells manage this acid load by measuring intracellular pH (pHi) in non-transformed osteoblasts in response to weak acid or bicarbonate loading. Basal pHi in mineralizing osteoblasts was ∼ 7.3 and decreased by ∼ 1.4 units upon replacing extracellular Na(+) with N-methyl-D-glucamine. Loading with 40 mM acetic or propionic acids, in normal extracellular Na(+), caused only mild cytosolic acidification. In contrast, in Na(+) -free solutions, weak acids reduced pHi dramatically. After Na(+) reintroduction, pHi recovered rapidly, in keeping with Na(+) /H(+) exchanger (NHE) activity. Sodium-dependent pHi recovery from weak acid loading was inhibited by amiloride with the Ki consistent with NHEs. NHE1 and NHE6 were expressed strongly, and expression was upregulated highly, by mineralization, in human osteoblasts. Antibody labeling of mouse bone showed NHE1 on basolateral surfaces of all osteoblasts. NHE6 occurred on basolateral surfaces of osteoblasts mainly in areas of mineralization. Conversely, elevated HCO 3- alkalinized osteoblasts, and pH recovered in medium containing Cl(-), with or without Na(+), in keeping with Na(+) -independent Cl(-) /HCO 3- exchange. The exchanger AE2 also occurred on the basolateral surface of osteoblasts, consistent with Cl(-) /HCO 3- exchange for elimination of metabolic carbonate. Overexpression of NHE6 or knockdown of NHE1 in MG63 human osteosarcoma cells confirmed roles of NHE1 and NHE6 in maintaining pHi. We conclude that in mineralizing osteoblasts, slightly basic basal pHi is maintained, and external acid load is dissipated, by high-capacity Na(+) /H(+) exchange via NHE1 and NHE6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.