ArticleBiomedical optics express2011
Detection and monitoring of the multiple inflammatory responses by photoacoustic molecular imaging using selectively targeted gold nanorods.
Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 46 citations in OpenAlex.
- Emerging Hybrid Intracoronary Imaging Technologies and Their Applications in Clinical Practice and Research.JACC. Cardiovascular interventions · 2024Review
- Nanoparticles for Thrombus Diagnosis and Therapy: Emerging Trends in Thrombus-theranostics.Nanotheranostics · 2024Review
- Photostable, hydrophilic, and near infrared quaterrylene-based dyes for photoacoustic imaging.Materials science & engineering. C, Materials for biological applications · 2018Article
- Article
- Molecular Imaging of Vulnerable Atherosclerotic Plaques in Animal Models.International journal of molecular sciences · 2016Review
- Gold nanoparticles for photoacoustic imaging.Nanomedicine (London, England) · 2015Review
- Combination-targeting to multiple endothelial cell adhesion molecules modulates binding, endocytosis, and in vivo biodistribution of drug nanocarriers and their therapeutic cargoes.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Echographic imaging of tumoral cells through novel nanosystems for image diagnosis.World journal of radiology · 2014Review
- Photoacoustic Imaging for Cancer Detection and Staging.Current molecular imaging · 2013Article
- Ferritin as a novel reporter gene for photoacoustic molecular imaging.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- Noninvasive molecular imaging of neuroinflammation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2012Review
- Transgene delivery using poly(amino ether)-gold nanorod assemblies.Biotechnology and bioengineering · 2012Article
- Echographic detectability of optoacoustic signals from low-concentration PEG-coated gold nanorods.International journal of nanomedicine · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
In vitro cell experiments have been performed to detect and monitor the upregulation of intercellular adhesion molecule-1 (ICAM-1) and E-selectin simultaneously by photoacoustic molecular imaging (PMI). Human umbilical vein endothelial cells (HUVECs) were grown on gelatin-coated glass slides and stimulated with inflammatory cytokines to induce the expression of the inflammatory biomarkers, ICAM-1 and E-selectin. Gold nanorods (GNRs) of aspect ratio (AR) 1:3 with absorption centered at 715 nm conjugated to anti-ICAM-1 antibody and GNRs of AR 1:3.5 with absorption centered at 800 nm conjugated to anti-E-selectin were exposed to HUVECs with different stimulation conditions. A focused high frequency ultrasonic transducer (60 MHz, f/1.5) was used to scan the photoacoustic (PA) signal over the top surface of the cell containing slides. Averaged PA signal intensity from the stimulated cells was about 3 folds higher (~10 dB) compared to the un-stimulated cells for both ICAM-1 and E-selectin. The strong binding of GNRs to the stimulated HUVEC cells was evidenced by fluorescence imaging. Exposure of HUVEC cells to GNRs conjugated to isotype control antibodies confirms a low level non-specific binding. Also, at 0, 2, 6, and 24 hours after inflammatory stimulation, the HUVECs were exposed to GNRs conjugated anti-ICAM-1 antibody and anti-E-selectin antibody. PA intensity at each stage of inflammation compares well with fluorescence imaging and rt-PCR quantification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.