ArticlePloS one2011
Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Predominant miRNAs in Animal-Source Foods and Bioinformatic Analysis.Current issues in molecular biology · 2026Article
- Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome forBiology · 2022Article
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- The emerging regulatory roles of noncoding RNAs in immune function of fish: MicroRNAs versus long noncoding RNAs.Molecular genetics and genomics : MGG · 2021Review
- Molecular Mechanisms Regulating Muscle Plasticity in Fish.Animals : an open access journal from MDPI · 2020Review
- Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole.International journal of molecular sciences · 2020Article
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- Low expression levels of plasma miR-141 are associated with susceptibility to gastric cancer.Oncology letters · 2019Article
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- Fishing Into the MicroRNA Transcriptome.Frontiers in genetics · 2018Review
- Differential miRNA expression in the three-spined stickleback, response to environmental changes.Scientific reports · 2017Article
- Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing.Malaria journal · 2016Article
- Temperature during early development has long-term effects on microRNA expression in Atlantic cod.BMC genomics · 2015Article
- MicroRNA in teleost fish.Genome biology and evolution · 2014Review
- Characterization of novel precursor miRNAs using next generation sequencing and prediction of miRNA targets in Atlantic halibut.PloS one · 2013Article
- In vivo screening of zebrafish microRNA responses to bacterial infection and their possible roles in regulating immune response genes after lipopolysaccharide stimulation.Fish physiology and biochemistry · 2012Article
- Identification of common carp (Cyprinus carpio) microRNAs and microRNA-related SNPs.BMC genomics · 2012Article
- Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development.BMC genomics · 2012Article
- Postprandial regulation of hepatic microRNAs predicted to target the insulin pathway in rainbow trout.PloS one · 2012Article
- Analysis of the Asian seabass transcriptome based on expressed sequence tags.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes. So far miRNAs have been only identified in a few fish species, although there are over 30,000 fish species living under different environmental conditions on the earth. Here, we described an approach to identify conserved miRNAs and characterized their expression patterns in different tissues for the first time in a food fish species Asian seabass (Lates calcarifer). METHODOLOGY/PRINCIPAL
findingsBy combining a bioinformatics analysis with an approach of homolog-based PCR amplification and sequencing, 63 novel miRNAs belonging to 29 conserved miRNA families were identified. Of which, 59 miRNAs were conserved across 10-86 species (E value ≤ 10⁻⁴) and 4 miRNAs were conserved only in fish species. qRT-PCR analysis showed that miR-29, miR-103, miR-125 and several let-7 family members were strongly and ubiquitously expressed in all tissues tested. Interestingly, miR-1, miR-21, miR-183, miR-184 and miR-192 showed highly conserved tissue-specific expression patterns. Exposure of the Asian seabass to lipopolysaccharide (LPS) resulted in up-regulation of over 50% of the identified miRNAs in spleen suggesting the importance of the miRNAs in acute inflammatory immune responses. CONCLUSIONS/SIGNIFICANCE: The approach used in this study is highly effective for identification of conserved miRNAs. The identification of 63 miRNAs and determination of the spatial expression patterns of these miRNAs are valuable resources for further studies on post-transcriptional gene regulation in Asian seabass and other fish species. Further identification of the target genes of these miRNAs would shed new light on their regulatory roles of microRNAs in fish.
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