ArticlePloS one2011
Drop-on-demand single cell isolation and total RNA analysis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Acoustic Bioprinting: A Glimpse Into an Emerging Field.Small methods · 2026Review
- Fate of Gold Nanoparticles in Laser Desorption/Ionization Mass Spectrometry: Toward the Imaging of Individual Nanoparticles.Journal of the American Society for Mass Spectrometry · 2023Article
- Emulating Human Tissues and Organs: A Bioprinting Perspective Toward Personalized Medicine.Chemical reviews · 2020Review
- 3-D Microwell Array System for Culturing Virus Infected Tumor Cells.Scientific reports · 2016Article
- Recent advances in bioprinting techniques: approaches, applications and future prospects.Journal of translational medicine · 2016Review
- Engineering cancer microenvironments forMaterials today (Kidlington, England) · 2015Article
- Multiscale assembly for tissue engineering and regenerative medicine.Trends in biotechnology · 2015Review
- Single-cell printing based on impedance detection.Biomicrofluidics · 2015Article
- Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Engineering anisotropic biomimetic fibrocartilage microenvironment by bioprinting mesenchymal stem cells in nanoliter gel droplets.Molecular pharmaceutics · 2014Article
- 25th anniversary article: Rational design and applications of hydrogels in regenerative medicine.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- Microfluidic chip-based technologies: emerging platforms for cancer diagnosis.BMC biotechnology · 2013Review
- Manipulating biological agents and cells in micro-scale volumes for applications in medicine.Chemical Society reviews · 2013Review
- Population transcriptomics with single-cell resolution: a new field made possible by microfluidics: a technology for high throughput transcript counting and data-driven definition of cell types.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Bioprinting for stem cell research.Trends in biotechnology · 2013Review
- Integrating nanoscale technologies with cryogenics: a step towards improved biopreservation.Nanomedicine (London, England) · 2012Article
- Prediction and control of number of cells in microdroplets by stochastic modeling.Lab on a chip · 2012Article
- Single cell genome sequencing.Current opinion in biotechnology · 2012Review
- Nanoliter droplet vitrification for oocyte cryopreservation.Nanomedicine (London, England) · 2012Article
- Emerging technologies for assembly of microscale hydrogels.Advanced healthcare materials · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Technologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and patterning of single cells from heterogeneous solutions as well as to keep them alive during the process due to a limited degree of control over single cell manipulation. Here, we present a microdroplet based method to isolate and pattern single cells from heterogeneous cell suspensions (10% target cell mixture), preserve viability of the extracted cells (97.0±0.8%), and obtain genomic information from isolated cells compared to the non-patterned controls. The cell encapsulation process is both experimentally and theoretically analyzed. Using the isolated cells, we identified 11 stem cell markers among 1000 genes and compare to the controls. This automated platform enabling high-throughput cell manipulation for subsequent genomic analysis employs fewer handling steps compared to existing methods.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.