ArticleNature protocols2011
Expression microdissection adapted to commercial laser dissection instruments.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2022Article
- Utilization of Laser Capture Microdissection Coupled to Mass Spectrometry to Uncover the Proteome of Cellular Protrusions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions.International journal of molecular sciences · 2019Article
- Computer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.Journal of pathology informatics · 2018Article
- Optimized expression-based microdissection of formalin-fixed lung cancer tissue.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- Article
- Extensive rewiring of epithelial-stromal co-expression networks in breast cancer.Genome biology · 2015Article
- SIVQ-LCM protocol for the ArcturusXT instrument.Journal of visualized experiments : JoVE · 2014Article
- Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection.Analytical chemistry · 2013Article
- Validation of esophageal squamous cell carcinoma candidate genes from high-throughput transcriptomic studies.American journal of cancer research · 2013Article
- Integrating contextual miRNA and protein signatures for diagnostic and treatment decisions in cancer.Expert review of molecular diagnostics · 2011Review
- SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling.Journal of pathology informatics · 2011Article
- Multiplex quantitative measurement of mRNAs from fixed tissue microarray sections.Applied immunohistochemistry & molecular morphology : AIMMReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Laser-based microdissection facilitates the isolation of specific cell populations from clinical or animal model tissue specimens for molecular analysis. Expression microdissection (xMD) is a second-generation technology that offers considerable advantages in dissection capabilities; however, until recently the method has not been accessible to investigators. This protocol describes the adaptation of xMD to commonly used laser microdissection instruments and to a commercially available handheld laser device in order to make the technique widely available to the biomedical research community. The method improves dissection speed for many applications by using a targeting probe for cell procurement in place of an operator-based, cell-by-cell selection process. Moreover, xMD can provide improved dissection precision because of the unique characteristics of film activation. The time to complete the protocol is highly dependent on the target cell population and the number of cells needed for subsequent molecular analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.